In this issue:
Original papers
Selection and citological analysis of culture filtrate Gaeumannomyces graminis var. tritici resistant cellular lines of wheat and regenerants from them
Bavol A.V., Dubrovna O.V., Lyalko I.I. P.191-200
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The culture filtrate G. graminis var. tritici resistant cellular strains were selected, and regenerated plants were obtained. The cytogenetical peculiarities of callus culture in cultivation on the selective medium where studied. Cytological analysis of the regenerants, obtained from resistant cellular strain, revealed plants with different levels of ploidy.
Key words: Triticum aestivum L., cellular selection, culture filtrate of Gaeumannomyces graminis var. tritici, cytogenetical analysis.
Study of genetic aspects of human reproductive disorders
Zastavna D.V., Terpylyak O.I., Huleyuk N.L., Tyrkus M.Ya. P.201-208
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The final results tell us about the essential influence of the genetic part in infertility. In the primary infertility group the chromosome quantity changes prevailed and in secondary – structural. It was observed a significant rising of the unstable chromosomes aberration and in 8,5 % - microdeletions in Y-chromosome in males with spermatogenesis infringement. It was determined, that immunogenetic markers of secondary infertility, caused by accustomed miscarriages in I trimester were HLA-antigens A10, B41 and B38. It was confirmed, that presence of common HLA-antigens in marital couples and increase of high expression GG-genotupes SNP –1082 G>A пІЛ–10 was prognostically unfavourable for running of pregnancy. It is expected, that IL-10 gene may be active in pathogenesis of accustomed miscarriages.
Key words: infertility, recurrent miscarriage, chromosomes aberration, microdeletions in Y-chromosome, HLA-system, single nucleotide polymorphism (SNP), genes of immune answer.
Expression of forming process in winter wheat hybrids for group resistance against main plant diseases when using ACIB in forest-steppe of Ukraine
Kyrylenko V.V. P.209-215
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Series of crosses of parental forms differing in resistance level was carried out at the V.M.Remeslo Myronivka Institute of Wheat for creation of winter wheat breeding material with group resistance against causal agent of main leaf diseases when using ACIB of pathogens. In hybrid combinations inheritance of the trait depended on maternal cytoplasm, therefore in crosses for resistance against group of diseases variety with group resistance should be involved as maternal form.
Key words: winter wheat, group resistance, hybrid combinations, plant diseases, hybridization, inheritance, transgression.
Influence of gene AN3 on the form of leaves Arabidopsis thaliana (l.) Heynh.
Kirpicheva I.V. P.216-223
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It was stated, that sheet plates rosette leaves of a mutant an3-1 back-lanceolate, rarer oblong form, while a wild type leaves of oval form. Form of stem leaves of plants line an3-1 oblong form, unlike an oval form a wild type.
Key words: arabidopsis, heteropfylly, gene AN3, form of leaves.
Cloning and heterological expression of Streptomyces nogalater IMET43360 O-methyltransferase genes, involved in nogalamycin biosynthesis
Klymyshyn D.O., Luzhetskyy A.M., Gromyko O.M., Myrashchenko L.R., Fedorenko V.O. P.224-232
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The snogM and snogL genes were subcloned from nogalamycin biosynthesis cluster. A set of plasmid constructions, caring snoM and snoL fragments, were used for heterological gene expression in the S. еchinatus and S. peucetius strains. These vectors were transferred into aranciamycin and doxorubicin producers using bacterial conjugation. It was found that S. еchinatus pKC1218EsnogM strain produced a novel compound. This fact is a result of the aranciamycin molecule modification by the expression of snogM gene in S. еchinatus.
Key words: Streptomyces nogalater, nogalamycin, heterological expression, methyltransferases.
Head diseases of winter wheat from forest-steppe of Ukraine
Kovalyshyna H.M., Murashko L.A., Kovalyshyn A.B.P.233-239
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Results of studying specific composition of pathogens of winter wheat head and seeds are given. Resulted from mycological analyze it was determined that mycoflora of attacked ears consists of such fungi species as Mucor mucedo, Penicillium viridicatum, P. expansum, Alternaria alternata, A. tenussima, Cladosporium herbarum, Fusarium sporotrichiella, F. oxysporum, F. moniliforme, F. culmorum, F. graminearum, F. gibbosum. F. graminerum was the most widespread species among Fusarium genus. Comparatively resistant against these disease cultivars Demetra, Rostyslavna, Bagira, Snizhana were singled out.
Key words:winter wheat, head, grain, fungi, infection, incidence, resistance, susceptibility.
Molecular-genetic analysis of genes associated with economically useful traits in domestic pig (Sus scrofa)
Konoval О.M., Kostenko S.О., Spyrydonov V.G., Melnichuk S.D. P.240-245
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The genetic analysis of Large White pigs breed of Ukraine was made by 10 genes of economically important traits by PCR RFLP. It was find out desirable alleles of all researched genes associated with reproductive traits, growth Rate and resistance to colibacteriosis.
Key words: ESR, FSHB, MYF4, PRLR, MC4R, CMyc, RYR, FUT1, MUC4, MYOD1, Large White pigs.
About the problem of unification of terminology during elaboration of androclinal haploidy biotechnology in spring soft wheat
Kruglova N.N.P.246-255
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By the position of experimental plant embryology the using terminology as the methodological base of elaboration of innovation biotechnology of spring soft wheat androclinal haploidy has been analyzed. The some terms have been proposed.
Key words: Triticum aestivum L., spring soft wheat, plant embryology, androclinia, anther culture in vitro, biotechnology.
Reseach of nucleotide sequence of plasmid pSS27
Lukyanchuk V.V., Polishchuk L.V., Matselyukh B.P.P.256-261
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It was found significant homology (up to 92%) of Streptomyces sequences of hybrid plasmid рSRL13, рSRL16 (F-primer) and pSW49 (R- primer) with ones of genes for sporulation protein of Streptomyces coelicolor A3(2) and S. avermitilis MA-4680 chromosomes. Also it is established them homology (73 %) with sequences of TraА-genes some Streptomycetes plasmids (pJV1, рSLS and pSN22). Homology one to others of sequences of plasmid pSS27 which were maintenanced in hybrid plasmids рSRL13, рSRL16 (F-primer) and pSW49 (R- primer) was established.
Key words: Streptomycetes. plasmid, nucleotide sequence.
Reproductive parametres of the budgerigars, if birds with rare mutations of the plumage colour are used for reproduction
Markova O.A. P.262-268
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Crossings between normal budgerigars and birds with rare mutations of the plumage colour: greywing, cinnamon and spangle, – have been analyzed. It has been shown, that use of the mutant birds does not change significantly average quantity of the nestlings in the clutch. It has been found, that the average quantity of the eggs in the clutch in the control group is 6,90. Significant increase of the eggs quantity in the clutch to 8,83 has been observed in the crossings between greywing males and normal heterozygous females, to 8,47 in the crossings between normal heterozygous males, and cinnamon females, and also to 8,35 in the crossings between cinnamon males and normal females.
Key words: budgerigar, mutations of the plumage colour, reproductive parameters.
Application of bioanalyzer 'Agilent 2100' for molecular-genetic fingerprinting of biological objects
Pankratov V.S., Uhimchuk A.N., Filipenya V.L., Spiridovich E.V.P.269-274
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A characteristic to Bioanalyzer Agilent 2100 is given. Its operating principle is described and its advantages are compaired with agarose gel electrophoresis separation of DNA fragments are mentioned. Possibilities of using Bioanalyzer in studies involving genetic fingerprinting is discussed.
Key words:Bioanalyzer, molecular-genetic marking, RAPD-and ISSR-markers.
Heat-dependent activity of guaiacol peroxidase in Apx2 knock-out mutants of Arabidopsis
Pyrizhok R.Yu., Volkov R.A., Panchuk I.I. P.275-281
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Changes in guaiacol peroxidase (POD) activity upon heat shock were tested wild type plants of Arabidopsis and in two lineages of ascorbate peroxidase 2 (Apx2) knock-out mutants. After 2 hours of moderate heat treatment (37°C) 27-36% increase of POD activity was found; no significant difference between the wild type plants and knock-out mutants was observed. Upon severe heat shock the effect was better pronounced: after 2 hours treatment at 44°C POD activity was 60 and 77% higher in two lineages of knock-out mutants but only 16% higher in wild type plants comparing to the control probes incubated at room temperature. The data indicate that POD appears to be involved in the heat stress response and can functionally compensate the loss of APX activity.
Key words::Arabidopsis thaliana, ascorbate peroxidase, guaiacol peroxidase, heat shock, knock-out mutants.
Generation of Echium plantagineum L. callus culture – producent of shikonin
Poronniк О.О., Kuchma M.D., Кunakh V.А.P.282-286
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E. plantagineum tissue culture has been generated from the roots of seed shoots on 5C01 agarized nutrient medium. Cultivation on the LS-m nutrient medium induced biosynthesis of the red shikonin pigments. Variant of callus tissue (3Ep), accumulating 8.6% of shikonin derivatives per dry biomass whose yield on the 14th day of growth constituted up to 16.8 mg/L of nutrient medium was selected. The total 3Ер callus productivity of shikonin derivatives to 14th day of growth constituted up to 1.4 g/L and 2.5 g/L of nutrient medium under extended to 20th day of passage.
Key words: Echium plantagineum L., plant tissue culture, red pigments, shikonin, cell lines, producents of biologically active substances.
Spring barley germplasm for selection for fungi diseases resistance
Sabadyn V.Ya. P.287-294
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The paper presents results of expression of resistance against causal agents of powdery mildew, stripe leaf blotch, Helmithosporium leaf blotch, dwarf leaf rust of barley, and main agronomic traits in spring barley collection samples. Spring barley samples resistance against causal agent of powdery mildew under laboratory and field conditions was ascertained. Variety samples Europrestige and Jacobe having complex of agronomic traits were singled out. The best collection samples are recommended for breeding to develop new high productive and disease resistant cultivars.
Key words: spring barley, powdery mildew, leaf blotch, Helminthosporium leaf blotch, resistance, races, agronomic traits.
Growth pecularities of soybean cell lines on cultural media with ions – inhibitors of nitrate reductase
Sergeeva L.E., Mikhalskaya S.I., Tishchenko E.N.P.295-301
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On the selective medium with toxic concentration of tungstate oxyanions the resistant cell lines of soybean cv.Kievskaya-27 were obtained. The selected clones demonstrate their resistance more than five years. It was investigated the growth of W-resistant lines during their cultivations on various selective media: with the addition of tungstane or vanadium oxyanios and potassium chlorate. These lines retained the normal growth under stress pressure of any agent. The clones proliferation on selective media with only nitrate form of the nitrogen is a possible mark of the selection of the new type of the nitrate reductase.
Key words: soybean, tungstate , vanadium – oxyanions, potassium chlorate, resistance, nitrate reductase.
Receiving and studying of the multimutant line of Arabidopsis thaliana (L.) Heynh. with attributes going beyond familyspecific
Sokolov I.D., Medved’ O.M. P.302-309
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By crossing of triple mutants, the subsequent selection in F2 and F3 and duplication mutant lines ap1,bp,clv1,gl1 have been received, which united in itself the mutant attributes of parents forms. Tetramutant recombinant certainly have mutational changes beyond diagnostic (species-, genera- and familyspesific) attributes.
Key words: arabidopsis, multimutant line, genotype, phenotype.
Proteomic approach for identificatoin of differentially expressing DNA-binding proteins of Secale cereale seedlinds
Spiridovich E.V., Vlasova A.B., Reshetnikov V.N. P.310-318
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A study of DNA-binding protein fraction of rye cultivar “Verasen'” by means of 2-D electrophoresis with subsequent trypsin hydrolysis of differentially expressed protein spots and MALDI TOF detection was undertaken. Data, showing the influence of microwave-irradiation treatment of seeds on the DNA-binding protein expression, including HSP-70, were obtained.
Key words: microwave radiation, fraction of DNA-binding proteins, 2-D electrophoresis, MALDI-TOF-TOF mass spectrometry.
Creation of resistant against powdery mildew and septoria blotch winter bread wheat initial material by experimental mutagenesis
Khomenko S.О. P.319-325
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The efficiency of using mutagens on winter wheat hybrids with the purpose of creation of resistant against pathogens forms was studied. It was ascertained the treatment of hybrid seeds with mutagens enabled to obtain winter wheat hybrid-mutant forms with increased resistant against powdery mildew and septoria leaf blotch.
Key words: winter wheat, hybrids, mutagenesis, powdery mildew, septoria blotch.
Different level bioindication of individual territories under intensive radio-ecological monitoring in Belarus
Chizhyk O.V., Bulko O.P., Reshetnikov V.N.P.326-336
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The aim of this work is to study the possibility of physiology-biochemical changes in plants, caused by radio-ecological effect, for elaboration a system of bioindication of territories with heightened radio-ecological capacity.
Key words: radiation, polydeoxyribonucleitide, radionuclide, gel, chlorophyle, DNA.
Reviews
The epigenome in evolution: beyond the modern synthesis
Jablonka E., Lamb M.J. С.337-355
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The pages of history
Once more about Lysenko
Syvolap Yu.M, Maliuta S.S. С.356-363
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Paper presents response to attempts at rehabilitating of T. Lysenko and “lysenkivshchyna". It appraises “lysenkivshchyna" as a kind of totalitarian regime in science leadership and replacement of the latter with pseudoscientific ersatz acceptable for the ruling political regime.
Key words:science history, history of science in USSR, genetics and breeding in Ukraine, “lysenkivshchina”.
Anniversaries
Academician A.O.Sapegin as a founder of scientific basics for plant breeding (to 125th anniversary of the birthday)
С.364-366
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Information
I International conference "Drozophila in experimental genetics and biology"
С.367-369
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IV International conference "Agents for experimental evolution of organisms"
С.369-373
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V International conference "Plant genome"
С.373-376
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