Genetic profiling of bacteria belongs to genus Pseudomonas, what affects legumes

  • L. A. Dankevich
DOI: https://doi.org/10.7124/FEEO.v22.935

Abstract

Aim. For the purpose of correct inter species identification and estimation of group’s heterogeneity, the genome fingerprinting of isolated by us and collection "Pseudomonas lupini" strains as well as typical representative of genus, affecting legumes, has been carried out. Methods. In the course of research, microbiological, molecular genetic (REP-PCR) methods and method of molecular phylogenetics (UPGMA) were used. Results. The genetic heterogeneity of isolated and collections "Pseudomonas lupini" strains has been estimated. A relationship between isolated and collections "Pseudomonas lupini" strains with the typical Pseudomonas syringae strains, affecting legumes, for BOX, REP and ERIC profiles has been determined. Conclusions. BOX, ERIC and REP-profiling of the genome of the agent of lupines’ brown spottiness revealed significant genetic heterogeneity of its population (from 20 to 50 % of heterogeneity) and close similarity of this pathogen to representatives of the species Pseudomonas syringae.
Keywords: identification, genetic heterogeneity, REP-PCR, causative agent of lupines’ brown spottiness.

References

Patyka V.P. Phytopathogenic Bacteria in Contemporary Agriculture. Microbiologichny zhurnal. 2016. Vol. 78(6). P. 71–83.

Patyka W., Gnatiuk T., Zhytkevych N., Kalinichenko A. Occurrence of the pathogenic bacteria Pantoea agglomerans in soybean cultivation. Progress in Plant Protection. 2015. Vol. 55(3). P. 280−285.

Zhy`tkevy`ch N.V., Novoxacz`ky`j L.M., Dankevy`ch L.A., Gnatyuk T.T. Curtobacterium flacumfaciens − novy`j zbudny`k zaxvoryuvannya soyi v Ukrayini. XII z’yizd Tovary`stva mikrobiologiv Ukrayiny` im. S.M. Vy`nogracz`kogo: tezy` dopovidej (Uzhgorod, 25–30 trav. 2009 r.). Uzhgorod: Uzhgorods`ky`j nacional`ny`j universy`tet, 2009. P. 303.

Dankevy`ch L.A. Fenoty`pni ta genoty`pni vlasty`vosti zbudny`ka buroyi bakterial`noyi plyamy`stosti lyupy`nu. My`kroby`ol. zhurn. 2006. Vol. 68(6). P. 20–27.

Bel`tyukova K.Y`, Koroleva Y`.B., Muras V.A. Baktery`al`nye bolezny` zerno – bobovy`x kul`tur. K.: Naukova dumka, 1974. 39 p.

Gvozdyak R.I., Pasichny`k L.A., Yakovleva L.M., Moroz S.M., Ly`tvy`nchuk O.O., Zhy`tkevy`ch N.V., Xodos S.F., Bucenko L.M., Dankevy`ch L.A., Gry`nny`k I.V., Paty`ka V.P. Fitopatogenni bakteriyi. Bakterial`ni xvoroby` rosly`n. K.: TOV "NVP "Interservis", 2011. 444 p.

Brenner D.J, Krieg N.R., Staley J.T., Garrity G.M. Bergey’s Manual of Systematic Bacteriology. New York USA: Springer Science+ Business Media, 2005. 689 p.

Louws F.J., Rademaker J.L.W., de Bruijn F.J. The three DS of PCR−based genomic analysis of phytobacteria: Diversity, Detection, and Disease Diagnosis. Annual Reviews Phytopathology. 1999. Vol. 37. P. 81−125.

Louws F.J., Fulbright D.W., Stephens C.T. Specific genomic fingerprints of phytopathogenic Xanthomonas and Pseudomonas pathovars and strains generated with repetitive sequences and PCR. Applied and Enviromental Microbiology. 1994. Vol. 60(7). P. 2286−2295.