The growth and the development of plantlets in Kalanchoe daigremontiana (Raim.-Hamet & Perrier) A. Berger under the influence of izatison components, the composition of the solvents DMSO and PEG 400

  • V. A. Katsan Institute of Molecular Biology and Genetics, NAS of Ukraine, Ukraine, 03143, Kyiv, Akad. Zabolotnogo str., 150 https://orcid.org/0000-0002-9972-4353
  • A. I. Potopalsky Institute of Molecular Biology and Genetics of NAS of Ukraine, Ukraine, 03143, Kyiv, Akademika Zabolotnogo str., 150
  • B. O. Zadorozhnii Institute of Molecular Biology and Genetics of NAS of Ukraine, Ukraine, 03143, Kyiv, Akademika Zabolotnogo str., 150
Keywords: Izatison, composition of DMSO and PEG 400, Kalanchoe Daigremontiana, development and growth of plantlets

Abstract

Aim. Research of the adaptive morphogenesis in Kalanchoe Daigremontiana uder the action of Izatison components, the composition of the solvents DMSO and PEG 400 (D+P). Methods. The leaves of Kalanchoe plants of the same age and origin, grown in laboratory condition by the method of soil culture, were used for the investigation. For D+P, the same dilutions values as in previous study for Izatisone were chosen. Results. The possible impact of D+P as stressors, which are inherent in causing a significant range of variability regarding the initiation, development and growth of the plantlets in Kalanchoe Daigremontiana, was revealed. Depending of the D+P concentration and of the individual susceptibility of the kalanchoe plants, D+P showed the ability to both stimulate and inhibit the development and growth of the plantlets, but in most cases such effects were not statistically significant. Conclusions. Hence, the stimulatory effect of Izatison on the appearance of plantlets in Kalanchoe Daigremontiana and their morphogenesis and growth, revealed by us earlier, is due, apparently, to N-methyl isatin ß-thiosemicarbazone, a derivative of isatin, which is a known phytohormone.

References

Katsan V. A., Potopalsky A. I., Zadorozhnii B. O. Influence of Izatison on growth and development of Plantlets in Kalanchoe Daigremontiana (Raim.-Hamet & Perrier) A. Berger. Factors in Experimental Evolution of Organisms. 2024. Vol. 35. P. 129–134. https://doi.org/107124/FEEO/V.35.1672. [in Ukrainian]

Zaika L. A., Bolsunova O. I., Potopalsky А. І. Antiviral, antitumor and immunomodulatory properties of the medicinal preparation Izatison. Kyiv : Kolobig, 2010. 211 p. [in Ukrainian]

Katsan V. A., Yurkevich L. N., Potopalsky A. I. Izatison and nanosilver are able to induce the changes in growth and productivity of oat plant cultivar Nezlamny persisting in the next generations. Factors in Experimental Evolution of Organisms. 2015. Vol. 16. P. 114–119. [in Ukrainian]

Kharina A. V., Kot T. G., Polischuk V. P., Zaets I. E., Chervatyuk N. V., Potopalsky A. I. Izatison as inhibitor of plant virus infections. Microbiology and Biotechnology. 2009. 2. P. 158–162. [in Ukrainian]

Potopalsky A. I., Katsan V. A., Yurkevich L. N., Lozyuk L. V. Method of increase of plant productivity and disease resistance: Patent for utility model 38265 Ukraine. No u200810756; applied on 28.08.2008, published on 25.12.2008, bulletin № 24. [in Ukrainian]

Katsan V. A., Potopalsky A. I., Zadorozhnii B. O. Influence of Izatison concentration on the growth and the grain productivity of the oat cultivar Nezlamni. Grail of Science. 2021. 5. P. 73–80. [in Ukrainian]

Galston A. W., Chen H. R. Auxin Activity of Isatin and Oxindole-3-Acetic Acid. Plant Phy. 1965. Vol. 40 (4). P. 699–705. https://doi.org/10.1104/pp.40.4.699.

Kutacek M., Galston A. W. The metabolism of 14C-labeled Isatin and Anthanilate in Pisum stem sections. Plant Phy. 1968. Vol. 43 (11). P. 1793–1798. https://doi.org/10.1104/pp.43.11.1793.

Mano Y., Nemoto K. The pathway of auxin biosynthesis in plants. J. Exp. Bot. 2012. Vol. 63 (8). P. 2853–2872. https://doi.org/10.1093/jxb/ers091.

Martynenko O. I., Kyrylenko T. K., Stepanyugin A. V., Plodnik D. P., Hovorun D. M. Quantitative estimation of genetically determined response in wheat leaves to the impact of chemical factors. Ukrainica Bioorganica Acta. 2013. Vol. 11 (2). P. 21–24. [in Ukrainian]

Sessa G., Carabelly M., Sassi M. The Ins and Outs of Homeodomain-Leucine Zipper/Hormone Networks in the Regulation of Plant Development. Int. J. Mol. Sci. 2024. Vol. 25 (11). 5657. https://doi.org/10.3390/ijms25115657.

Yue Wu, Lin Shi, Lei Li, Liwen Fu, Yanlin Liu, Yan Xiong, Jen Sheen Integration of nutrient, energy, light, and hormone signal-ling via TOR in plants. J Exp Bot. 2019. Vol. 70 (8). P. 2227–2238. https://doi.org/10.1093/jxb/erz028.

Liu H., Blanford J., Shi H., Schwender J., Shancling J., Zhai Z. The target of rapamycin kinase is a positive regulator of plant fatty acid and lipid synthesis. Plant Physiol. 2025. Vol. 197 (2). kiae639. https://doi.org/10.1093/plphys/kiae639.

D’alessandro S., Velay F., Lebrun R., Zafirov D., Mehrez M., Romand S., Saadouni R., Forzani C., Yterne S., […] Field B. Posttranslational regulation of photosynthetic activity via the TOR kinase in plants. Science Advances. 2024. Vol. 10 (25). eadj3268. https://doi.org/10.1126/sciadv.adj3268.

Tuncer S., Gurbanov R., Sheraj I., Solel I., Esenturk O., Banerjee S. Low dose dimethyl sulfoxide driven gross molecular changes have the potencial to interfere with various cellular processes. Sci. Reports. 2018. Vol. 8 (1). 14828. https://doi.org/10.1038/s41598-018-33234-z.

Sajid M., Ahmed S., Sardar R., Ali A., Yasin N. A. Role of polyethylene glycol to alleviate lead stress in Raphanus sativus. Peer J. 2025. Vol. 13. e18147. http://doi.org/10.7717/peerj.18147.

Zhao Q., Xiong H., Yu H., Wang C., Zhang S., Hao J., Wang J., Zhang H., Zhang L. Function of MYB8 in larch under PEG simulated drought. Scientifc Reports. 2024. Vol. 14:112190. https://doi.org/10.1038/s41598-024-61510-8.

Garcês H. M. P., Koenig D., Townsley B. T., Kim M., Sinha N. R. Truncation of LEAFY COTYLEDON1 protein is required for asexual reproduction in Kalanchoë daigremontiana. Plant Physiology. 2014. Vol. 165 (1). P. 196–206. https://doi.org/10.1104/pp.114.237222.

Zhu Ch., Wang L., Chen J., Liu Ch., Zeng H., Wang H. Over-expression of KdSOC1 gene affected plantlet morphogenesis in Kalanchoe daigremontiana. Scientific Reports. 2017. Vol. 7 (1). 5629. https://doi.org/10.1038/s41598-017-04387-0.