Differentiation of accessions from the national lentil collection according to the level of drought resistance on a various concentrations PEG-6000 solution

  • N. Vus
  • А. О. Vasylenko
  • L. M. Shevchenko
DOI: https://doi.org/10.7124/FEEO.v29.1409

Abstract

Aim. assessment  of  accessions from the lentil national collectionon the level of drought resistance on a PEG-6000 solution. Methods. The germination was carried out in growth plates on a filter paper bed at t = 21ºC for five days. A working solution of PEG-6000 with concentrations of 15.0% and 19.5% was used. The control was germinated in distilled water. The length of the shoot and root was measured and the level of depression of growth processes was calculated. Results. When germinating on a PEG-6000 solution with 19.5% concentration, the overwhelming number of accessions had depression indicators for shoots above 85% and above 60% for roots, and therefore it was not possible to isolate samples tolerant to the action of PEG-6000. When germinating on a 15%  solution of PEG-6000, a wider range of variation was noted: depression in feature of “root length” ranged from 0% to 100%, “shoot length” - from 50% to 100%. The calculated Spearman's rank correlation coefficient confirms that shoots, in comparison with roots, are more sensitive to the action of osmotic in both variants of the experiment. Conclusions. The concentration of PEG-6000 was established for the selection of sources and the ranking of samples by drought resistance. Sources of drought resistance were identified from the studied sample – accessions subsp. microsperma: 8-MIL M 5 (UD0600007, France), UD0600028 (Ethiopia), Giza 9 (UD0600065, Egypt) and the accession-standard of drought resistance is Gornostepnaya (UD0600131, Armenia) subsp. microsperma.

Keywords: lentil, PEG-6000, concentration, sources of drought resistance.

References

FAOSTAT, 2021. Retrieved from: http://www.fao.org/faostat/en/#data/QC.

Sichkar V.I., Kryvenko A.I., Solomonov R.V. Lantil in world and Ukraine: current state and prospect. Journal of Native and Alien Plant Studies. 2020. Vol. 16. P. 178–193. doi: 10.37555/2707-3114.16.2020.219830.

Dash A.P., De D.K., Mohanty S., Lenka D. Screening of Lentil (Lens culinaris Medik.) Genotypes and Correlation Analysis under PEG Imposed Water Stress Condition. International Journal of Bio-resource and Stress Management. 2017. Vol. 8 (4). P. 539–547. doi: 10.23910/ijbsm/2017.8.4.1829c.

Koskosidis A., Khah E., Mavromatis A., Pavli O., Vlachostergios D. Effect of PEG-induced drought stress on germination of ten chickpea (Cicer arietinum L.) genotypes. Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2020. Vol. 48 (1). P. 294–304. doi: 10.15835/nbha48111799.

Florido M., Bao L., Lara R.M., Castro Y., Acosta R., Álvarez M. Effect of water stress simulated with PEG 6000 on tomato seed germination (Solanum section Lycopersicon) Cultivos Tropicales. 2018. Vol. 39 (1). Р. 87–92. Retrieved from: http://ediciones.inca.edu.cu/index.php/ediciones/article/download/1429/pdf.

Vus N., Vasylenko A., Lutenko V., Kobyzeva L., Besuhla O., Shevchenko L., Ponurenko S., Feng Baili, Saliy D. Concentration effect of polyethylene glycol in evaluation of grain legumes for drought tolerance. Žemės Ūkio Mokslai. 2020. Vol. 27 (2). Р. 149–159.

Kobyzeva L.N., Bezugla O.M., Sylenko S.I., Kolotylov V.V., Sokol T.V., Dokukina K.I., Vasylenko A.O., Bezuglyi I.M., Vus N.O. Methodical recommendations for studying the genetic resources of legumes. NAAS of Ukraine. Plant Production Institute named after V.Ya. Yuriev. Kharkiv, 2016. 84 p. [in Ukrainian]