Comparative cytogenetic analysis of early reproductive losses depending of maternal age

  • I. R. Tkach
  • N. L. Huleyuk
  • D. V. Zastavna
  • K. O. Sosnina
  • G. M. Bezkorovaina
  • N. V. Helner
DOI: https://doi.org/10.7124/FEEO.v23.1019

Abstract

Aim. Human reproduction characterized by a high incidence of aneuploidies. Approximately 99 % of conceptions with anomalies of karyotype terminate of pregnancy loss, mainly during the first 14 weeks of intrauterine development. The frequency and spectrum of karyotype anomalies in the chorionic villus of early reproductive losses were studied depending of maternal age. Methods. Banding cytogenetic and interphase mFISH with the centromeric probe panel for chromosomes 13, 14, 15, 16, 17, 18, 21, 22, X and Y were used. Results. The contribution of different karyotype abnormalities in the genesis of the early reproductive losses depends on the age of the woman, namely, with age significantly increases the proportion of aneuploidy due to autosomal trisomies and reduced contribution of polyploidy and gonosomal monosomy. The main autosomal trisomy in the material of lost pregnancies from women under the age of 35 is 16, 21 and 15, 22, 13 and 18 in order of decreasing frequency, and from older women 16, 15, 22, 21, 13 and 14. Conclusions. The structure and rate of karyotype anomalies in the material of lost pregnancies varies with maternal age.

Keywords: early reproductive loss, maternal age, karyotype abnormalities.

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