Activity of sucrose metabolizing enzymes at the milk-waxy ripeness endosperm of maize inbred lines T2-plants transformed in planta using LBA4401 (pBi2E)

Abstract

Aims. Comparative studying the activity of sucrose metabolizing enzymes of T2-plants 250, 370, 1555 transformed in planta by disarmed strain LВА4404 harboring vector constructions pBi2E was investigated. Methods. Sucrose’s metabolism enzymes activity and carbohydrates’ content under effect of the Agrobacterium-mediated transformation by strain LВА4404 were measured. Results. The alterations of the sucrose’s and hexoses’ metabolism at the endosperm of the T2 plant generation milk-waxy ripeness corn seeds were occurred under Agrobacterium-mediated transformation on the plants’ pollination stage. Conclusions. The data indicate genotypic changes in the sucrose’s metabolism enzymes activity – sucrose synthase and invertase, as well as at the carbohydrates’ content under effect of the Agrobacterium-mediated transformation by strain LBA4404.

Key words: Zea mays L., transformation, sucrose synthase, invertase carbohydrates’ content, endosperm, T2-plants.