Obtaining the transgenic hairy root culture of topinambour (Helianthus tuberosus L.) containing hu-man interferon alpha-2b gene

Abstract

The aim of the study was to obtain Helianthus tuberosus L. hairy root culture containing human interferon alpha gene driven by constitutive 35S CaMV and root specific Mll sugar beet promoters by means of Agrobacterium rhizogenes-mediated transformation. Results. We observed the high level callus and Ri-roots induction on A. rhizogenes–inoculated leaf and stem explants on the selective medium in 2-3 week cultivation. PCR proved the presence of human interferon alpha gene for the studied samples as well as it showed the obtained hairy root culture of the transgenic origin. Conclusions. Thus we manage to obtain the transgenic H. tuberosus hairy root culture containing human interferon alpha gene.

Key words: Helianthus tuberosus L., human interferon alpha-2b, Agrobacterium rhizogenes-mediated transformation, tissue-specific promoters, hairy root and transgenic callus culture.