Генетичне профілювання патогенних для яблуні штамів Erwinia sp.
Анотація
Aim. It is known, that significant damage to pome fruit causing representatives of phylogenetically closely related species Erwinia amylovora, E. pyrifoliae, E. piriflorinigrans, E. uzenensis. That is why, for their correct identification using molecular genetic techniques, including REP-PCR, which can not only correctly identify the pathogen, and evaluate the heterogeneity of its population. In this regard, the aim of our research was genome fingerprinting of isolated strains and typical Erwinia amylovora UCM B 1095Т strain, using REP-PCR, for their correct taxonomy at the species level. Methods. Genetic profiling of isolated Erwinia sp. strains and the typical Erwinia amylovora B 1095T strain carried out by REP-PCR. Results. As a result of REP-PCR analysis revealed a significant relationship of isolated Erwinia sp. strains and the typical Erwinia amylovora UCM B 1095T strain. Conclusions. As a result, BOX, REP and ERIC profiling set low genetic heterogeneity of isolated Erwinia sp. strains and their great affinity with the typical Erwinia amylovora UCM B 1095T strain.
Keywords: REP-PCR, isolated Erwinia sp. strains, Erwinia amylovora, pome fruit culture.
Посилання
Hvozdiak R.I., Pasichnyk L.A., Yakovleva L.M., Moroz S.M., Lytvynchuk O.O., Zhytkevych N.V., Khodos S.F., Butsenko L.M., Dankevych L.A., Hrynnyk I.V., Patyka V.P. Fitopatohenni bakterii. Bakterial'ni khvoroby roslyn / Za red. V.P. Patyky. Kyuv: TOV NVP ‘Interservis’, 2011. 444 p. [in Ukrainian]
Perelik rehul'ovanykh shkidlyvykh orhanizmiv [Elektronnyy resurs]. Ministerstvo ahrarnoi polityky Ukrainy. Nakaz No 467 vid 4 serpnia 2010 r. 2010. Retrieved from: http://zakon2.rada.gov.ua/laws/show/z0720-10. [in Ukrainian]
Kim W.S., Gardan L., Rhim S.L., Geider K. Erwinia pyrifoliae sp. nov., a novel pathogen that affects Asian pear trees (Pyrus pyrifolia Nakai). Iternational Journal of Systematic Bacteriology. 1999. V.49. P. 899–906.
Lopez Marıa M., Rosello M., Llop P., Ferrer S., Christen R., Gardan L. Erwinia piriflorinigrans sp. nov., a novel pathogen that causes necrosis of pear blossoms. Iternational Journal of Systematic Bacteriology. 2011. V.61. P. 561–567. doi: 10.1099/ijs.0.020479-0.
Matsuura T., Mizuno A., Tsukamoto T., Shimizu Y., Saito N., Sato S., Kikuchi S., Uzuki T., Azegami K., Sawada H. Erwinia uzenensis sp. nov., a novel pathogen that affects European pear trees (Pyrus communis L.). Iternational Journal of Systematic Bacteriology. 2012. V. 62. P. 1799–1803. doi: 10.1099/ijs.0.032011-0.
Barionovi D., Giorgi S., Stoeger A.R, Ruppitschand W., Scortichini M. Characterization of Erwinia amylovora strains from different host plants using repetitive-sequences PCR analysis, and restriction fragment length polymorphism and short-sequence DNA repeats of plasmid pEA29. Journal of Applied Microbiology. 2006. V. 100. P. 1084–1094. doi: 10.1111/j.1365-2672.2006.02813.x.
McManus P.S., Jones A. Genetic fingerprinting of Erwinia amylovora strains isolated from tree-fruit crops and Rubus spp. [Electronic resource]. The American phytopathological society. 1995. Retrieved from: https://www.apsnet.org/publications/phytopathology/backissues/Documents/1995Articles/Phyto85n12_1547.PDF.
Dankevych L.A., Votselko S.K., Shcherbyna T.M., Patyka V.P. Fenotypova heterohennist' fitopatohennykh bakteriy rodu Erwinia – zbudnykiv khvorob iabluni v Ukraini. Mikrobiol. zhurn. 2016. V. 78(5). P. 30–41. [in Ukrainian]
Teixeira A.C.O., Marques A.S.A., Ferreira M.A.S.V. Low genetic diversity among pathogenic strains of Erwinia amylovora from Brazil. Braz. J. Microbiol. 2009. V. 40. P. 678–684. doi.org/10.1590/S1517-83822009000300033.
Rico A., Fuhrer E., Ortiz-Barredo A., Murillo J. Polymerase chain reaction fingerprinting of Erwinia amylovora has a limited phylogenetic value but allows the design of highly specific molecular markers. Phytopathology. 2008. V. 98. P. 260–269. doi: 10.1094/ PHYTO-98-3-0260.
Krasniqi N., Muslui A., Lepaja K., Lepaja L. Assessment of the sanitary status of pome fruit crops in Kosovo, with particular emphasis to the bacterial disease the Fire Blight [Electronic resource]. Proceedings. 50th Croatian and 10th International Symposium on Agriculture. Opatija. Croatia. 2015. Retrieved from: http://sa.agr.hr/pdf/2015/sa2015_p0911.pdf.
