Эффекты SNP CAPN316 и CAST282 на количественные характеристики потомства быков пород молочного и мясного направлений
Анотація
Aim. The aim of the study was to analyze effects of SNPs CAPN316 in CAST282 in calpain and calpastatin genes on offspring productive traits produced by dairy and beef bulls. Methods. Data on offspring productivity included milk performance traits (for dairy bulls), birth weight and average daily gain (for beef bulls). Molecular genetic analysis was performed by PCR-RFLP. χ²-, t-tests and Pearson correlation coefficient were used for statistical analysis at significance levels of 0.05. Results. In progeny of dairy bulls number of alleles C in both SNPs demonstrated negative correlation with milk yield (r=-0.577), fat yield (r=-0.794) and protein yield (r =-0.798). G allele of CAPN316 (“wild type”) was associated with increased number of beef bulls progeny. Body weight and average daily gain was better in GG-bulls offspring. Conclusions. C alleles of SNPs CAPN316 in CAST282 being associated with meat quality traits were found to be ineffective in selection for milk production traits.
Keywords: calpain and calpastatin genes, SNP, CAPN316, CAST282, bull’s genotype, evaluation on progeny, offspring productivity.
Посилання
Hill R., Canal A., Bondioli K., Morell R., Garcia M.D. Molecular markers located on the DGAT1, CAST, and LEPR genes and their associations with milk production and fertility traits in Holstein cattle [Elektronnyy resurs]. Genet. Mol. Res. 2016. 8 p. Retrieved from: http://www.funpecrp.com.br/gmr/year2016/vol15-1/pdf/gmr7794.pdf.
Collis E., Fortes M.R.S., Zhang Y., Tier B., Schutt K., Barendse W., Hawken R. Genetic variants affecting meat and milk production traits appear to have effects on reproduction traits in cattle. Anim. Genet. 2012. V. 43(4). P. 442–446. doi: 10.1111/j.1365-2052.2011.02272.x.
Washburn S.P., Silvia W.J., Brown C.H., McDaniel B.T., McAllister Trends in reproductive performance in Southeastern Holstein and Jersey DHI herds. J. Dairy Sci. 2002. V. 85(1). P. 244–251. doi: 10.3168/jds.S0022-0302(02)74073-3.
Miquel M.C., Villarreal E., Mezzadra C., Melucci L., Soria L., Corva P., Schor A. The association of CAPN1 316 marker genotypes with growth and meat quality traits of steers finished on pasture. Genet. Mol. Biol. 2009. V. 32(3). P. 491–496. doi: 10.1590/S1415-47572009000300011.
Schenkel F.S., Miller S.P., Jiang Z., Mandell I.B., Ye X., Li H., Wilton J.W. Association of a single nucleotide polymorphism in the calpastatin gene with carcass and meat quality traits of beef cattle. J. Anim. Sci. 2006. V. 84(2). P. 291–299.
Garcia M.D., Michal J.J., Gaskins C.T., Reeves J.J., Ott T.L., Liu Y., Jiang Z. Significant association of the calpastatin gene with fertility and longevity in dairy cattle. Anim. Genet. 2006. V. 37. P. 304–305. doi: 10.1111/j.1365-2052.2006.01443.x.
Lysenko N.G., Kolesnik A.I., Goraychuk I.V., Ruban S.Iu., Fedota A.M. Association of calpain and calpastatin genes with exterior traits of aberdeen-angus. Faktory eksperimental'noy evoliutsii organizmov. 2016. V. 18. P. 111–116. [in Russian]
Kataloh buhaiv molochnykh i molochno-m’iasnykh porid dlia vidtvorennia matochnoho poholiv’ia v 2014 rotsi. Kyiv, 2014. [in Ukrainian]
Kataloh buhaiv molochnykh i molochno-m’iasnykh porid dlia vidtvorennia matochnoho poholiv’ia v 2015 rotsi. Kyiv, 2015. [in Ukrainian]
Atramentova L.O., Utievs'ka O.M. Statystychni metody v biolohii. Kharkiv: KhNU im. V.N. Karazina, 2007. 288 p. [in Ukrainian]
Hrebinyk D.M. Strukturno-vydovi osoblyvosti kal'painiv iak osnova ikh klasyfikatsii. Fizyka zhyvoho. 2012. V. 20(1–2). P. 4–8. [in Ukrainian]
Curi R.A., Palmieri D.A., Suguisawa L., de Oliveira H.N., Silveira A.C., Lopes C.R. Growth and carcass traits associated with GH1/Alu I and POU1F1/Hinf I gene polymorphisms in Zebu and crossbred beef cattle. Genet. Mol. Biol. 2006. V. 29(1). P. 56–61. doi: 10.1590/S1415-47572006000100012.
