Генетичний аналіз успадкування жирних кислот соняшнику (Helianthus annuus L.)
Анотація
Aim. The definition of some peculiarities of inheritance of fatty acid content in the seed oil of the source material and the creation of sunflower lines and hybrids with improved quality of oil composition and economic traits’ complex is an important issue. Methods. The genetic control of palmitic, stearic, oleic, linoleic fatty acids of sunflower with the use of the joint scaling test (the test Cavalli) has been studied. To establish the type of genes’ interaction the model with 3 and 6 genetic parameters is applied and for their estimation in the analysis there are involved the average data of traits on 6 generations. Results. It has been shown that the additive-dominant model has not proved to be true in case of fatty acids inheritance. It was possible to reveal an interaction between genes by duplicate epistasis type for palmitic and oleic acids, complementary epistasis type – for linoleic acid. The interaction type was not possible to establish as some model parameters have insufficient resolving power for stearic acid. Conclusions. Discovered differently directed effect of genes, which leads to increase or decrease the phenotypic manifestation of traits. Therefore it is possible the selection of optimal breeding pairs to create sunflower hybrids with different fatty acid composition of the oil.
Keywords: Helianthus annuus L., test Cavalli, inheritance, fatty acids.
Посилання
Gavrilova V.A., Anisimova I.N. Genetika kul'turnykh rasteniy. Podsolnechnik. Sankt-Peterburg: VIR, 2003. 209 p. [in Russian]
Sharypina Ia.Iu., Popov V.N., Kirichenko V.V. Analiz stsepleniia genov, kontroliruiushchikh fermenty u podsolnechnika. Genetika. 2007. V. 43(11). P. 1486-1490. [in Russian] doi: 10.1134/S1022795407110063
Chen J., Hu J., Vick B., Jan C. Molecular mapping of a nuclear male-sterility gene in sunflower (Helianthus annuus L.) using TRAP and SSR markers. Theor. Appl. Gen. 2006. V. 113. P. 122-127. doi: 10.1007/s00122-006-0278-2
Mokrani L., Gentzbittel L., Azanza F., Fitamant L., Al-Chaarani G., Sarrafi A. Mapping and analysis of quantitative trait loci for grain oil content and agronomic traits using AFLP and SSR in sunflower (Helianthus annuus L.). Theor. Appl. Gen. 2002. V. 106. P. 149-156. doi: 10.1007/s00122-002-1011-4
Ternovskaia T.K. Khromosomnaia lokalizatsiia glavnykh genov kolichestvennykh priznakov (QTL) pshenitsy s ispol'zovaniem genov-markerov D khromosom. Tsitologiia i genetika. 2000. V. 34(2). P. 16-24. [in Russian]
Leon A., Lee M., Andrade F. Quantitative trait loci for growing degree days to flowering and photoperiod response in sunflower (Helianthus annuus L.). Theor. Appl. Genet. 2001. V. 102. P. 497-503. doi: 10.1007/s001220051673
Mather K., Jinks J.L. Biometrical genetics. London, 1971. 382 p. doi: 10.1007/978-1-4899-3404-8
Tarutina L.A., Khotyleva L.V. Vzaimodeystvie genov pri geterozise. Minsk: Nauka i tekhnika, 1990. 176 p. [in Russian]
Brahin O.M. Stvorennia vykhidnoho materialu ta hibrydiv soniashnyku z pidvyshchenym vmistom hlitserydiv pal'mitynovoi kysloty v olii: avtoref. dys. na zdobuttia stupenia kand. s.-h. nauk: spets. 06.01.05 «Selektsiia roslyn». Kharkiv, 2010. 20 p. [in Ukrainian]
Fernández-Martínez J., Jimenez A., Dominguez J., Garcia J., Garces R., Mancha M. Genetic analysis of the high oleic acid content in cultivated sunflower (Helianthus annuus L.). Euphytica. 1989. V. 41, Is. 1. Р. 39-51. doi: 10.1007/BF00022409
Miller J.F., Vick B.A. Registration of three low palmitic acid and five low stearic acid sunflower genetic stocks. Crop Sci. 1999. V. 39. Р. 305. doi: 10.2135/cropsci1999.0011183X003900010080x
Vick B.A., Jan C.C., Miller J.F. Two-year study on the inheritance of reduced saturated fatty acid content in sunflower seed. Helia. 2004. V. 27(41). Р. 25-40. doi: 10.2298/HEL0441025B
Pérez-Vich B., Garcés R., Fernández-Martínez J.M. Inheritance of medium stearic acid content in the seed oil of a sunflower mutant CAS-4. Crop Sci. 2002. V. 42. Р. 1806-1811. doi: 10.2135/cropsci2002.1806
Chastnaia selektsiia polevykh kul'tur / Pod red-ey Iu.B. Konovalova - Moskva: Agropromizdat, 1990. 544 p. [in Russian]
DSTU 10857-64 Nasinnia oliyne. Metody vyznachennia oliynosti. Vyznachennia skladu zhyrnykh kyslot v olii provodyt'sia metodom hazoridynnoi khromatohrafii. [in Ukrainian]
Atramentova L.A., Utevskaia O.M. Statisticheskie metody v biologii. Gorlovka, 2008. 248 p. [in Russian]
Kocherina N.V. Algoritmy ekologo-geneticheskogo uluchsheniia produktivnosti rasteniy: dis... kand. biol. nauk: 03.00.15. Sankt-Peterburg, 2009. 130 p. [in Russian]
