Визначення алельного стану генів Glu-1 в гібридних сім’ях, носіїв гена Gpc-B1 від Triticum turgidum ssp. dicoccoides
Анотація
Aim. The aim of our study was to analyze the hybrid families of generation F5 carrying gene Gpc-B1 of Triticum turgidum ssp. dicoccoides for the allelic state of Glu-1, to calculate 1000 kernel weight and to select families with the best set of alleles and performance. Methods. Polymerase chain reaction for molecular genetic analysis and calculation of the 1,000-kernel weight for wheat grain yields were used. Results. Determination of the three loci Glu-A1, Glu-B1, Glu-D1 shows that among 44 hybrid families it is possible to identify 16 most promising families that have the most valuable allelic variants. Furthemore, given the analysis of the 1,000-kernel weight, two families (#36 and 40) having the highest values can be selected among those 16 families. Conclusions. The results of study enable a comprehensive approach to the selection of progeny with the best genetic base, which will be used in breeding of soft winter wheat later on.
Keywords: Triticum aestivum L., gene Gpc-B1, Glu-1 loci, PCR, molecular markers.
Посилання
Larchenko K.A., Morhun B.V. Oznaky iakosti zerna pshenytsi ta metody ikh polipshennia. Fiziologiia i biokhimiia kul'turnykh rasteniy. 2010. V. 42, No 6. P. 463–474. [in Ukrainian]
Yasmeen F., Khurshid H., Ghafoor A. Genetic divergence for high-molecular weight glutenin subunits (HMW-GS) in indigenous landraces and commercial cultivars of bread wheat of Pakistan. Genetics and Molecular Research. 2015. V. 14 (2). P. 4829–4839. doi: 10.4238/2015.May.11.15
Atabaieva Kh.N., Massino I.V. Biologiia zernovykh kul'tur. Tashkent: Gos. nauch. izd. «Uzbekiston milliy entsiklopediiasi», 2005. 204 p. [in Russian]
Payne P.I., Law C.N., Mudd E.E. Control by homoeologous group 1 chromosome of the high molecular-weight subunits of glutenin, a major protein of wheat endosperm. Theor. Appl. Genet. 1980. V. 58. P. 113–120. doi: 10.1007/BF00263101
Payne P. Genetics of wheat storage proteins and the effect of allelic variation on bread-making quality. Ann. Rev. Plant Physiol. 1987. V. 38. P. 141–153. doi: 10.1146/annurev.pp.38.060187.001041
Payne P., Corfield K., Holt L., Blackman J. Correlations between the inheritance of certain high-molecular-weight glutenin subunits and bread-making quality in progenies of six crosses of bread wheat. J. Sci. Food. Agric. 1981. V. 32. P. 51–60. doi: 10.1002/jsfa.2740320109
Rybalka O.I., Lytvynenko M.A. Novitni henetychni aspekty polipshennia iakosti pshenytsi. Visn. ahrar. nauky. 2009. No 4. P. 35–39. [in Ukrainian]
Molots'kyy M.Ya., Vasyl'kivs'kyy S.P., Kniaziuk V.I., Vlasenko V.A. Selektsiia i nasinnytstvo sil's'kohospodars'kykh roslyn. Kyiv: Vyshcha osvita, 2006. 463 p. [in Ukrainian]
Zhemela H.P., Kuznetsova O.A. Vplyv sortovykh vlastyvostey na produktyvnist' ta iakist' zerna pshenytsi m’iakoi ozymoi. Sil's'ke hospodarstvo. Roslynytstvo. 2012. No 3. P. 23–25. [in Ukrainian]
Tseluyko O.A., Medvedeva V.I. Zavisimost' massy 1000 zeren sel's'kokhoziaystvenykh kul'tur ot udobreniy. Agronomiia i lesnoe khaziaystvo. 2014. No 2. P. 58–60. [in Russian]
Pokhylko S.YU., Troyanovska A.V., Stepanenko A.I., Urbanovich O.Yu., Dugan O.M., Rybalka О.І., Morhun B.V. Studies of bread wheat genotypes with transferred gene GPCB1 of Triticum turgidum ssp. dicoccoides. Faktory eksperymental'noi evoliutsii orhanizmiv. 2016. V. 18. P. 132–135. [in Ukrainian]
Stewart C.N., Via L.E. A rapid CTAB DNA isolation technique useful for RAPD fingerprinting and other PCR applications. BioTechniques. 1993. V. 14, No. 5. P. 748–749.
Stepanenko A.I. Rozrobka system molekuliarno-henetychnykh markeriv dlia detektsii iakisnykh oznak u pshenytsi ta iachmeniu : dys. kand. biol. nauk: 03.00.20. Kyiv, 2015. 164 p. [in Ukrainian]
DSTU 4138–2002. Nasinnia sil's'kohospodars'kykh kul'tur. Metody vyznachennia iakosti. Derzhspozhyvstandart Ukrainy. 2013. P. 172. [in Ukrainian]
Pokhyl'ko S.Yu., Morhun B.V., Stepanenko A.I., Duhan O.M. Analiz bilkovykh fraktsiy ozymoi pshenytsi nosiiv hena Gpc-B1 vid Triticum turgidum ssp. dicoccoides. Materialy I mizhnarodnoi naukovo-praktychnoi internet konferentsii «Biotekhnolohiia: dosvid, tradytsii ta inovatsii», 14–15 hrudnia 2016 r. Kyiv, 2016. P. 432. [in Ukrainian]
