Визначення ліній Drosophіla melanogaster, репортерна система яких чутлива до низьких доз мутагену
Анотація
Aim. There are many methods of assessment of mutagens, but the effects of low-dose mutagens have been overlooked. In this study we try to select lines of Drosophila melanogaster with high-sensitive to mutagen reporter gene. We used D. melanogaster lines with enhancer trap P {lacW} which produces beta-galactosidase gene in response to activation induced reparation. Methods. Ionizing radiation in dose of 100 roentgens were used as a mutagen. We extracted salivary glands of the third instar of the fruit fly larvae. The sample was fixed with 0.75 % glutaraldehyde and stained by 12-hour incubation with 0.2 % X-Gal at 37°C. Activity of the reporter gene was determined by microscope. Results. Two of the three lines are not shown according to the activation of the reporter gene from the resulting dose of ionizing radiation. In line y [1] w [67c23]; P {w [+ mC] = lacW} mus209 [k00704] / CyO (#10361) activation of the reporter gene occurred in some nuclei salivary gland after radiation. Conclusions. The reporter gene P{lacW} may be used for the detection of small doses of mutagens. Larvae of line #10361 has reporter gene activation in response to ionizing radiation.
Keywords: mutagenic factors, Drosophila melanogaster, LacZ reporter, radiation.
Посилання
Kakinuma S., Yamauchi K., Amasaki Y., Nishimura M., Shimada Y. Low-dose radiation attenuates chemical mutagenesis in vivo. J Radiat Res. 2009. Sep. V. 50 (5). P. 401–405.
Seifried H.E., Seifried R.M., Clarke J.J., Junghans T.B., San R.H. A compilation of two decades of mutagenicity test results with the Ames Salmonella typhimurium and L5178Y mouse lymphoma cell mutation assays. Chem Res Toxicol. 2006. May. V. 19 (5). P. 627–644.
Leme D.M., Marin-Morales M.A. Allium cepa test in environmental monitoring: a review on its application. Mutat Res. 2009. V. 682 (1). P. 71–81. doi: 10.1016/j.mrrev.2009.06.002.
Anderson D. Genetic and reproductive toxicity of butadiene and isoprene. Chem Biol Interact. 2001. V. 135–136. P. 65–80.
Au W.W., Badary O.A., Heo M.Y. Cytogenetic assays for monitoring populations exposed to environmental mutagens. Occup Med. 2001. V. 16 (2). P. 345–357.
Frenzilli G., Nigro M., Lyons B.P. The Comet assay for the evaluation of genotoxic impact in aquatic environments. Mutat Res. 2009. V. 681 (1). P. 80–92. doi: 10.1016/j.mrrev.2008.03.001.
Klapacz J., Pottenger L.H., Engelward B.P., Heinen C.D., Johnson G.E., Clewell R.A., Carmichael P.L., Adeleye Y., Andersen M.E. Contributions of DNA repair and damage response pathways to the non-linear genotoxic responses of alkylating agents. Mutat Res Rev Mutat Res. 2016. P. 767:77–91. doi: 10.1016/j.mrrev.2015.11.001.
Faucheux M., Netter S., Bloyer S., Moussa M., Boissonneau E., Lemeunier F., Wegnez M., Théodore L. Advantages of a P-element construct containing MtnA sequences for the identification of patterning and cell determination genes in Drosophila melanogaster. Mol Genet Genomics. 2001. V. 265 (1). P. 14–22.
