Формування мікробульб картоплі в культурі in vitro залежно від температури та інтенсивності освітленості

  • Ю. О. Лавриненко
  • Г. С. Балашова
  • В. В. Базалій

Анотація

Aim. To specify optimal techniques influencing the increase of intensity of potato tuber formation in meristem culture in vitro. Methods. Integrated use of laboratory, mathematical and statistical, calculation and comparison methods and the method of systematic analysis. Results. The paper represents experimental data about the influence of temperature and light intensity on the induction of tuber formation under microclonal reproduction of the health-improved initial material. It proves that the decisive factor in the process of morphogenesis of plants in vitro of the early maturing potato variety Kobza and their productivity is temperature conditions for cultivation. Conclusions. Optimal indexes of productivity and economic efficiency are maintained by growing test-tube plants under the temperature of cultivation of 14–16ºС and the light intensity of 3000 lux. The number of microtubers per plant was 1.2 pieces, the weight of an average microtuber was 262.0 mg, the weight of microtubers per plant was 363.7 mg, the number of microtubers weighing more than 350.0 mg was 20.9 %; the cost price of microtubers was 5.31 UAH with the profitability of 201 %.

Keywords: potato, microtubers, temperature mode, light intensity, in vitro.

Посилання

Zamalieva F.F., Safiullina G.F., Nazmieva R.R. Vnedrenie sistemy semenovodstva kartofelia na ozdorovlennoy osnove v Respublike Tatarstan. Kartofelevodstvo v regionakh Rossii : aktual'nye problemy nauki i praktiki / VNIIKKh. Moskva, 2006. P. 167–181. [in Russian]

Gareev R.G., Zamalieva F.F., Zaynulina A.S. Semenovodstvo – na ozdorovlennuiu meristemnuiu osnovu. Kartofel' i ovoshchi. 2001. No 1. P. 9–10. [in Russian]

Almasi A., Harsanyi A., Gaborjanyi R. Photosintetic aleration of virus infected plants. Acta Phytopathol Acad. 2001. V. 36, N 1-2. P. 15–29.

Kravchenko D.V., Uskov A.I., Varitsev Iu.A. [i dr.] Vozmozhnosti ispol'zovaniia immunokhromatograficheskikh test-sistem dlia diagnostiki virusov kartofelia. Sb. nauch. tr. «Kartofelevodstvo»: materialy koordinats. soveshch. i nauch.-prakt. konf., posviashch. 120-letiiu so dnia rozhdeniia A.G. Lorkha. M.: RASKhN, VNIIKKh, 2009. P. 208–213. [in Russian]

Anisimov B.V., Oves E.V., Topisheva O.V. [i dr.] Sovershenstvovanie virusologicheskogo kontrolia v protsesse formirovaniia i podderzhaniia banka zdorovykh sortov kartofelia. Sb. nauch. tr. «Kartofelevodstvo»: materialy koordinats. soveshch. i nauch.-prakt. konf., posviashch. 120-letiiu so dnia rozhdeniia A.G. Lorkha. M.: RASKhN, VNIIKKh, 2009. P. 188–192. [in Russian]

Sologub A.S., Mel'nik P.A. Sposob biokhimicheskoy identifikatsii sortov kartofelia. Sb. nauch. tr. «Kartofelevodstvo»: materialy koordinats. soveshch. i nauch.-prakt. konf., posviashch. 120-letiiu so dnia rozhdeniia A.G. Lorkha. M.: RASKhN, VNIIKKh, 2009. P. 178–182. [in Russian]

Zhuk V.N., Oliynyk T.N., Emets A.I. [i dr.] Agrobakterial'naia transformatsiia sortov kartofelia ukrainskoy selektsii SRY-genami, obespechivaiushchimi ustoychivost' k nasekomym-vrediteliam. Kartofelevodstvo : sb. nauch. tr. Minsk, 2008. V. 14. P. 67–73. [in Russian]

Awan A.R. Mughal S.M. In vitro elimination of potato leaf roll polerovirus from potato varieties. European Journal of Scientific Research. 2007. V. 18, N 1. P. 155–164.

Ramirez-Malagon R., Perez-Moreno L., Borodanenko A. Differential organ infection studies, potyvirus elimination and field performance of virus-free garlic plants produce by tissue culture. Plant Cell Tiss Organ Cult. 2006. V. 86. P. 103–110.

Buhaieva I.P., Snihovyy V.S., Kul'tura kartopli na pivdni Ukrainy. Kherson, 2002. 176 p. [in Ukrainian]

Bondarchuk A.A. Naukovi osnovy nasinnytstva kartopli v Ukraini. Bila Tserkva, 2010. 400 p. [in Ukrainian]

Kutsenko V.S., Osypchuk A.A., Podhaiets'kyy A.A. [ta in.] Metodychni rekomendatsii shchodo provedennia doslidzhen' z kartopleiu / In-t kartopliarstva. Nemishaieve, 2002. 183 p. [in Ukrainian]

Murashige T., Skoog F. A revised medium for rapid grown and bio-assays with tobacco tissue cultures. Physiol. Plant. 1962. V. 15. P. 473–497.