Селекція на гліфосаті трансгенних калюсних ліній кукурудзи генотипів, районованих в Україні

  • І. О. Нітовська Інститут клітинної біології та генетичної інженерії НАН України, Україна, 03143, м. Київ, вул. Акад. Заболотного, 148
  • І. К. Комарницький Інститут клітинної біології та генетичної інженерії НАН України, Україна, 03143, м. Київ, вул. Акад. Заболотного, 148
  • Б. В. Моргун Інститут клітинної біології та генетичної інженерії НАН України, Україна, 03143, м. Київ, вул. Акад. Заболотного, 148
DOI: https://doi.org/10.7124/FEEO.v20.771

Анотація

Aim. Glyphosate selection has a number of advantages over other commonly used selectable markers for maize. There is some natural variability within maize germplasm for degree of sensitivity to glyphosate. We investigated the selective effect of glyphosate for production transgenic maize callus after Agrobacterium-mediated transformation among geno-types of Ukrainian plant breeding. Methods. Agrobacterium-mediated transformation, glyphosate selection in vitro, and PCR analysis were used to obtain transgenic maize callus and to confirm its status. Results. An efficient selectable marker system for production transgenic maize callus lines tolerant to herbicide glyphosate was proposed. Calluses of four maze genotypes of Ukrainian plant breeding and pCB135 vector containing CP4epsps gene were used in Agrobacterium-mediated transformation experiments. Three callus maize lines of DK267×PLS61 genotype containing CP4epsps gene were obtained. Conclusions. The use of glyphosate as a selective agent after Agrobacterium-mediated transformation proved to be effective for transgenic maize callus lines production containing the gene CP4epsps. The success of Agrobacterium-mediated transformation of maize callus strongly depended on the genotype of source ma-terial.

Keywords: Agrobacterium-mediated maize transformation, CP4epsps gene, glyphosate selection, PCR analysis.

Посилання

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