Исследование влияния светодиодного освещения на рост и развитие ряда лекарственных растений в условиях in vitro

  • Л. Г. Лёшина Институт клеточной биологии и генетической инженерии НАН Украины, Украина, 03143, г. Киев, ул. Академика Заболотного, 148
  • О. В. Булко Институт клеточной биологии и генетической инженерии НАН Украины, Украина, 03143, г. Киев, ул. Академика Заболотного, 148
  • Н. А. Пушкарова Институт клеточной биологии и генетической инженерии НАН Украины, Украина, 03143, г. Киев, ул. Академика Заболотного, 148
  • А. А. Петерсон Институт клеточной биологии и генетической инженерии НАН Украины, Украина, 03143, г. Киев, ул. Академика Заболотного, 148
  • Н. В. Кучук Институт клеточной биологии и генетической инженерии НАН Украины, Украина, 03143, г. Киев, ул. Академика Заболотного, 148

Анотація

Aim. Modern light-emitting diodes cover all of the visible range of the optical spectrum and with the right LEDs color groups combination any spectral composition can be achieved. Also, the use of different wavelength combinations can influence plants grows and development. The aim of the present work was to study the monochrome blue LED lighting effect on the morphologic characteristics of some medical plants during in vitro cultivation. Methods. Digitalis pur-purea L., Aerva lanata L. Juss. ex Schult. and Cathranthus roseus G. Don in vitro cultivated plants were used for monochrome blue LED lighting effect estimation. Spectrometric methods of photosynthetic pigments and flavonoid content estimation were used. Results. In vitro plants grows with 440–460 nm LED lighting causes adaptive morpho-physiological changes with pigments and flavonoids content decrease. An increase in shoots formation, plant grows deceleration and interstices shortening were also found. Conclusions. Obtained results suggest insufficiency of mono-chrome LED lighting and the need of optimal spectral composition determination for successful plants cultivation. Keywords: LED lighting, Digitalis purpurea L., Aerva lanata L. Juss. ex Schult., Cathranthus roseus G. Don, morpho-physiological changes.

Посилання

Bourget C.M. An introduction to light-emitting diodes. HortScience. 2008. V. 43. P. 1944-1946. doi: 10.21273/HORTSCI.43.7.1944

Morrow R.C. LED lighting in horticulture. HortScience. 2008. V. 43. P. 1947-1950. doi: 10.21273/HORTSCI.43.7.1947

Hoenecke M.E., Bula R.J., Tibbitts T.W. Importance of 'Blue' photon levels for lettuce seedlings grown under red-light-emitting diodes. HortScience. 2002. V. 27. P. 427-430. doi: 10.21273/HORTSCI.27.5.427

Brown C.S., Schuerger A.C., Sager J.C. Growth and photomorphogenesis of pepper plants under red light-emitting diodes with supplemental blue or far-red lighting. J. Amer. Soc. Hort. Sci. 1995. V. 120. P. 808-813. doi: 10.21273/JASHS.120.5.808

Kopsell D.A., Sams C.E., Morrow R.C. Blue wavelengths from LED lighting increase nutritionally important metabolites in specialty crops. HortScience. 2015. V. 50. P. 1285-1288. doi: 10.21273/HORTSCI.50.9.1285

Murashige I., Scoog F. A revised medium for rаpid growth and bioassays with tobacco tissue cultures. Physiol. Plantarum. 1962. V. 15(3). P. 473-497. doi: 10.1111/j.1399-3054.1962.tb08052.x

Lichtenthaler H.K. Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods in Enzymology. 1987. V. 148. P. 350-382. doi: 10.1016/0076-6879(87)48036-1

Gage T.B., Wendei S.H. Quantitative determination of certain flavonol 3-glycosides. Anal. Chem. 1950. V. 22. P. 708-711. doi: 10.1021/ac60041a028