Ефективність андрогенезу in vitro в культурі пиляків сортів та їхніх гібридів пшениці м’якої озимої різних генерацій
Анотація
Aim. To estimate the level of in vitro haploproduction androgenic ability of different generations populations (F1, F2) soft winter wheat hybrids of the same combination to maximize the use of biotechnology cereals. Methods. Obtaining of bread wheat double haploid lines by anther culture in vitro. The statistical methods. Results. The influence different generation of wheat hybrids on the processes of induction and regeneration in anther culture of winter bread wheat were studied. The 21 green plants-regenerants were received. Conclusions. The using of anthers of the second generation simple hybrids as donor material for the effective technology creation of doubled haploid of bread winter wheat was shown.
Keywords: wheat, anther culture in vitro, callus, regeneration, generation simple hybrids.
Посилання
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