Гени контролю темпів розвитку як компоненти системи регуляції стійкості Triticum aestivum L. до біотичного стресу за умов in vitro

  • O. О. Авксентьєва Харківський національний університет імені В. Н. Каразіна, Україна, 61022, м. Харків, майдан Свободи, 4; Київський національний університет імені Тараса Шевченка, Україна, 01601 м. Київ, вул. Володимирська, 64/13
  • Н. В. Терентьєва Харківський національний університет імені В. Н. Каразіна, Україна, 61022, м. Харків, майдан Свободи, 4
DOI: https://doi.org/10.7124/FEEO.v20.755

Анотація

Aim. The aim of our work is to research the influence of exometabolites phytopathogens of genus Fusarium on callus culture isogenic lines for genes PPD (NILs) of winter soft wheat. Methods. In the work used standard biotechnological and mycological methods. The influence of exometabolites phytopathogens g. Fusarium investigated, adding CF mi-cromycetes to MS culture medium in a ratio of 1:20, using transplants callus culture of isogenic lines of wheat. Growth index analyzed density of callus tissue and size of callus cells determined. Results. Established that the CF of Fusarium oxysporum significantly slows grows reaction of callus cultures and morphological structure callus NILs is changes. It is shown that the impact exometabolites phytopathogens has the opposite effect on cytological parameters (number and length callus cells) in the isolines differing pace of development in conditions in vivo. The culture filtrate F. oxysporum has more toxicity compared to exometabolites F. moniliforme. Conclusions. It is supposed that the genetic system controlling the pace of development and photoperiodic sensitivity Triticum aestivum L. in conditions in vivo indirectly determines the formation of resistance to biotic stress conditions in vitro.

Keywords: Triticum aestivum L., Fusarium oxysporum, Fusarium moniliforme, PPD genes, NILs, callus culture, growth index, resistance to phytopathogens.

Посилання

Bavol A.V., Dubrovnaia O.V., Lialko I.I. Selektsiia in vitro miagkoy pshenitsy na ustoychivost' k Gaeumannomyces graminis var. Tritici. Fiziologiia i biokhimiia kul't. rasteniy. 2009. V. 41(4). P. 314–321. [in Russian]

Gritsiuk N.V. Ustoychivost' sortov ozimoy pshenitsy k fuzarioznim infektsiiam pri raznykh srokakh porazheniia Karantin i zashchita rasteniy. 2013. No. 10. P. 1–3. [in Russian]

Cockram J., Jones H., Leigh F., O’Sullivan D., Powell W., Laurie D., Greenland A. Control of flowering time in temperate cereals: genes, domestication and sustainable productivity. J. Exp. Botany. 2007. V.58(6). P. 1231–1244. doi: 10.1093/jxb/erm042.

Beales J., Turner A., Griffiths S., Snape J.W., Laurie D.A. A Pseudo-Response Regulator is misexpressed in the photoperiod insensitive Ppd-D1a mutant of wheat (Triticum aestivum L.). Theor. Appl. Genet. 2007. V. 115. P. 721–733. doi: 10.1007/s00122-007-0603-4.

Kitagawa S., Shimada S., Murai K. Effect of Ppd-1 on the expression of flowering-time genes in vegetative and reproductive growth stages of wheat. Genes Genet. Syst. 2012. V. 87. P. 161–168. doi: 10.1266/ggs.87.161.

Khotyljova L., Kaminskaya L., Koren L. Influence of genetic systems of Vrn- and Ppd genes on the ecological adaptation of wheat and triticale. Biologija. 2002. No. 4. P. 45–48.

Fayt V.I., Fedorova V.R. Vliianie razlichiy genov Ppd na agronomicheskie priznaki pshenitsy. Tsitologiia i genetika. 2007. No. 6. P. 26–33. [in Russian]

Molekuliarno-geneticheskie i biokhimicheskie metody v sovremennoy biologii rasteniy. Moskva: BINOM. Laboratoriia znaniy, 2011. 487 p. [in Russian]

Bilay V.I. Metody eksperimental'noy mikologii. Kyiv: Naukova dumka, 1982. 560 p. [in Russian]

Barykina R.P., Veselova T.D., Deviatov A.G., Dzhalilova Kh.Kh., Iliina G.M., Chubatova N.V. Spravochnik po botanicheskoy mikrotekhnike. Osnovy i metody. Moskva: Izd-vo MGU, 2004. 312 p. [in Russian]

Atramentova L.A., Utevskaia O.M. Statisticheskie metody v biologii. Gorlovka, 2008. 247 p. [in Russian]

Avksentiieva O., Taran N. Drought resistance and productivity of wheat and soybean isogenic lines with different photoperiodic sensitivity. Eureka: Life sciences. 2016. V. 5 (5). P. 8–17. doi: 10.21303/2504-5695.2016.00226.

Avksentyeva O.A., Zhmurko V.V. Genes Ppd and Vrn as components of molecular genetic system of wheat regulation resistance (Triticum aestivum L.) to abiotic stress. Molecular Approaches in Plan Abiotic Stress. Eds R.K. Gaur and Pradeep Sharma. CRC Press, 2014. P. 1–20. doi: 10.1201/b15538-2.

Ivanov V.B. Kletochnye mekhanizmy rosta rasteniy. Moskva: Nauka, 2011. 104 p. [in Russian]

Avksentyeva O.A., Terentyeva N.V. Influence of exometabolites phytopathogens g. Fusarium on morpho-physiological characteristics of callus cultures of isogenic by genes VRN wheat lines. Plant Protection News. 2016. V.3 (89). P. 16–17.