Реконструкція філогенії кластерів генів біосинтезу глікопептидів

Анотація

Aim. In current study we have endeavored to analyze the phylogeny of 18 sequenced glycopeptide biosynthetic gene clusters. Methods. Standard methods of phylogenetic reconstruction were used in this research. Results. We have created sets of conservative genes, present in all selected clusters and analyzed their phylogeny. In most cases two main clades were formed on final trees. One clade always included genes from biosynthetic clusters of teicoplanin-like compounds, the second – genes from biosynthetic clusters of vancomycin-like compounds. Finally, we have built a multilocus phylogeny (MLP) of selected clusters. Once again, two groups of glycopeptide gene clusters could be recognized according to the results of MLP: biosynthetic clusters of teicoplanin-like compounds and biosynthetic clusters of vancomycin-like compounds. These groups do not correlate with accepted structural classification of glycopeptides. Biosynthetic cluster of unusual antibiotic feglymycin appeared to be closely related to biosynthetic clusters of teicoplanin-like compounds. Conclusions. Based on the obtained results we proposed a possible scenario of the evolution of glycopeptide biosynthetic gene clusters.
Keywords: gene cluster, glycopeptide antibiotic, phylogeny.