Вплив сахарози на експресію генів Apx за дії теплового стресу

  • І. І. Панчук Чернівецький національний університет імені Юрія Федьковича, Україна, 58012, м. Чернівці, вул. Коцюбинського, 2м
  • О. В. Череватов Чернівецький національний університет імені Юрія Федьковича, Україна, 58012, м. Чернівці, вул. Коцюбинського, 2м
  • Р. А. Волков Чернівецький національний університет імені Юрія Федьковича, Україна, 58012, м. Чернівці, вул. Коцюбинського, 2м

Анотація

Aim. Sucrose plays an important role as a signaling molecule that causes changes in gene expression. However, the effect of sucrose on gene expression of antioxidant enzymes is poorly understood. The purpose of our study was to investigate the effect of sucrose on the expression of the multigenic family Apx in Arabidopsis thaliana under heat stress conditions. Methods. Leaves of A. thaliana were exposed to heat stress at 37ºC in the presence or in absent of sucrose. Subsequently, mRNA levels were determined in using quantitative RT-PCR. Results. It was found that absence of sucrose in the incubation buffer resulted in a decrease of mRNA for most Apx genes, both in heat stress-treated samples and after incubation at room temperature. The strongest effect was shown for Apx2: without sucrose the level of mRNA under heat stress was 30 times lower. Conclusions. Obtained data showed that sucrose can perform a signaling role in regulating the stress response upon heat stress, and, in particular, enhance the temperature-dependent gene expression of Apx1 and Apx2.

Keywords: Arabidopsis thaliana, multigenic family Apx, heat stress, mRNA, qPCR.

Посилання

Sauer N. Molecular physiology of higher plant sucrose transporters. FEBS Letters. 2007. V. 581. Р. 2309–2317. doi: 10.1016/j.febslet.2007.03.048.

Wind J., Smeekens S., Hanson J. Sucrose: metabolite and signaling molecule. Phytochemistry. 2010. V. 71, No 14–15. P. 1610–1614. doi: 10.1016/j.phytochem.2010.07.007.

Hellmann H.A., Smeekens S. Sugar sensing and signaling in plants. Frontiers Plant Sci. 2014. V. 5. P. 113. doi: 10.3389/fpls.2014.00113.

Rolland F., Sheen J. Sugar sensing and signaling networks in plants. Biochemical Society Transaction. 2005. V. 33. P. 269–271. doi: 10.1042/BST0330269.

Loreti E., Poggi A., Novi G., Alpi A., Perata P. A genome-wide analysis of the effects of sucrose on gene expression in Arabidopsis seedlings under anoxia. Plant Physiol. 2005. V. 137. P. 1130–1138. doi: 10.1104/pp.104.057299.

Ho S.-L., Chao Y.-C., Tong W.-F., Yu S.-M. Sugar coordinately and differentially regulates growth- and stress-related gene expression via a complex signal transduction network and multiple control mechanism. Plant Physiol. 2001. V. 125, No 4. P. 877–890. doi: 10.1104/pp.125.2.877.

Couee I., Sulmon C., Gouesbet G. Involvement of soluble sugars in reactive oxygen species balance and responses to oxidative stress in plants. J. Exp. Bot. 2006. V. 57. P. 449–459. doi: 10.1093/jxb/erj027.

Cao Y.-Y., Yang M.-T., Chen S.-Y., Zhou Z.-Q., Li X., Wang X.-J., Bai J.-G. Exogenous sucrose influences antioxidant enzyme activities and reduces lipid peroxidation in water-stressed cucumber leaves. Biologia Plantarum. 2015. V. 59, No 1. P. 147–153. doi: 10.1007/s10535-014-0469-7.

Nishikawa N., Kato M., Hyodo H., Ikoma Y., Sugiura M., Yano M. Effect of sucrose on ascorbate level and expression of genes involved in the ascorbate biosynthesis and recycling pathway in harvested broccoli florets. J. Exp. Bot. 2005. V. 56. P. 65–72. doi: 10.1093/jxb/eri007.

Budzhak V.V. Biometriia. Chernivtsi: Ruta, 2013. 326 p. [in Ukrainian]

Panchuk I.I., Volkov R.A., Schoffl F. Heat stress- and heat shock transcription factor-dependent expression and activity of APX in Arabidopsis. Plant Physiol. 2002. V. 129, No 6. P. 838–853. doi: 10.1104/pp.001362.