Гетерозиготна делеція гена β-катеніну у ранньому кардіогенезі спричиняє затримку росту серця і порушує кінетику канонічного Wnt сигналінгу

  • О. Л. Пальчевська Інститут молекулярної біології і генетики НАН України, Україна, 03680, м. Київ, вул. Акад. Заболотного, 150
  • А. А. Хазєєва Харківський національний університет ім. В.Н. Каразіна, Україна, 61022, м. Харків, пл. Свободи, 4
  • В. В. Балацький Інститут молекулярної біології і генетики НАН України, Україна, 03680, м. Київ, вул. Акад. Заболотного, 150
  • Т. П. Рубан Інститут молекулярної біології і генетики НАН України, Україна, 03680, м. Київ, вул. Акад. Заболотного, 150
  • Л. Л. Мацевич Інститут молекулярної біології і генетики НАН України, Україна, 03680, м. Київ, вул. Акад. Заболотного, 150
  • О. О. Півень Інститут молекулярної біології і генетики НАН України, Україна, 03680, м. Київ, вул. Акад. Заболотного, 150
DOI: https://doi.org/10.7124/FEEO.v20.739

Анотація

Aim. In our present work, we have analyzed newborn and embryonic heart under the β-catenin haploinsufficiency. Methods. Beta-catenin conditional knockout mice were bred with α-MHC-Cre transgenic mice. In such way we generate the β-catenin haploinsufficient new born (P1-2) and embryonic hearts (E12,5 an E14,5) With rtPCR using we analyze the canonical WNT signalling kinetics in embryonic and newborn hearts. Namely we have analyzed the level of TСF-4, Axin2, c-Fos and CyclinD2 genes expression. Beside of this we have studied the γ-catenin gene expression un-der normal and β-catenin haploinsufficient conditions. Results. Cardiac β-catenin knockout leads attenuated newborn heart growth and associated with γ-catenin expression up-regulation. Canonical Wnt signalling activated in later cardiogenesis (E12,5-14,5) in WT heart and downregulated in newborns. Conclusions. We have shown the importance of canonical Wnt during later cardiogenesis. Thus β-catenin haploinsufficiency leads to violation of WNT kinetic in latter embryos and attenuated the heart growth.
Keywords: heart development, cardiogenesis, WNT signalling, β-catenin, γ-catenin.

Посилання

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