Ідентифікація ліній кукурудзи плазми ланкастер серед інших типів зародкової плазми за результатами SNP-аналізу

  • К. В. Деркач ДУ Інститут зернових культур НААН України, Україна, 49027, м. Дніпро, вул. В. Вернадського, 14
  • В. В. Борисова ДУ Інститут зернових культур НААН України, Україна, 49027, м. Дніпро, вул. В. Вернадського, 14
  • Т. М. Сатарова ДУ Інститут зернових культур НААН України, Україна, 49027, м. Дніпро, вул. В. Вернадського, 14; ДВНЗ Український державний хіміко-технологічний університет, Україна, 49005, м. Дніпро, пр. Гагаріна, 8
  • Б. В. Дзюбецький ДУ Інститут зернових культур НААН України, Україна, 49027, м. Дніпро, вул. В. Вернадського, 14
  • В. Ю. Черчель ДУ Інститут зернових культур НААН України, Україна, 49027, м. Дніпро, вул. В. Вернадського, 14
  • М. М. Федько ДУ Інститут зернових культур НААН України, Україна, 49027, м. Дніпро, вул. В. Вернадського, 14
DOI: https://doi.org/10.7124/FEEO.v20.734

Анотація

Aim. Lancaster is one of the most promising breeding germplasm for cultivation in Steppe Zone of Ukraine. The comparative analysis of maize Lancaster germplasm inbreds of Ukrainian breeding program with other types of germplasm on SNP-markers was carried out. Methods. Identification of maize inbreds of Lancaster germplasm among other types of germplasm was made according to the results of SNP-analysis using BDI-III panel and STRUCTURE software. Results. The genetic diversity of 91 maize inbreds was estimated. The investigated inbreds was divided into k=2 groups. Identified allelic states of the SNP-markers of BDI-III panel appeared to be not unique in Lancaster germplasm inbreds, however, it can help to distinguish them from others ones. Conclusions. SNP-analysis revealed the presence of two groups of allelic states of SNP-markers among 91 maize inbred lines. Inbreds which according to pedigree information belong to Lancaster germplasm at 96.9 % fall into group 2. As for average coefficient (Q) of belonging to group 2 Lan-caster inbreds significantly differed from Iodent and Mix inbreds. Lancaster inbreds from Ukrainian breeding program have certain set of specific allelic variants of markers of BDI-III panel which allows distinguishing from Iodent inbreds.
Keywords: maize, SNP-markers, Lancaster germplasm, STRUCTURE software, genetic diversity.

Посилання

Haydash O.L. Otsinka kombinatsiynoi zdatnosti za vrozhaynistiu zerna samozapylenykh simey S5 kukurudzy (Zea mays L.) zmishanoi zarodkovoi plazmy. Sortovyvchennia ta okhorona prav na sorty roslyn. 2016. No 1 (30). P. 62–66. [in Ukrainian]

Sokolov V.M., Varenik B.F., Piliugin A.S., Guzhva D.V. Selektsionnaia otsenka elitnykh samoopylennykh liniy kukuruzy iz osnovnykh geterozisnykh grupp zarodyshevoy plazmy. Genetika, selektsiia i tekhnologiia vozdelyvaniia kukuruzy. 1999. P. 92–96. [in Russian]

Mhoswa L., Derera J., Qwabe F.N.P., Musimwa T.R. Diversity and path coefficient analysis of Southern African maize hybrids. Chilean J. Agric. Res. 2016. V. 76 (2). P. 143–151. doi: 10.4067/S0718-58392016000200002

Fed'ko M.M. Rezul'taty druhoho tsyklu rekurentnoho doboru v populiatsii kukurudzy (Zea maize L.) sporidnenoi z henoplazmoiu Lancaster Oh43. Biul. In-tu sil's'koho hospodarstva stepovoi zony NAAN Ukrainy. 2012. No 2. P. 55–60. [in Ukrainian]

Troyer A.F. Temperate corn background, behavior, and breeding. CRC Press, 2000. 468 p. doi: 10.1201/9781420038569.ch14

Smith S.D., Murray S.C., Heffner E. Molecular analysis of genetic diversity in a Texas maize (Zea mays L.) breeding program. Maydica. 2015. V. 60. P. 1–8.

Olmos S.E., Lia V.V., Eyhérabide G.H. Genetic diversity and linkage disequilibrium in the Argentine public maize inbred line collection. Plant Genetic Resources. 2016. P. 1–12. doi: 10.1017/S1479262116000228.

Dziubets'kyy B.V., Bodenko N.A., Fed'ko M.M., Husak Yu.V. Stvorennia seredn'opiznikh hibrydiv kukurudzy na bazi plazmy Lankaster (S103). Biul. In-tu sil's'koho hospodarstva stepovoi zony NAAN Ukrainy. 2012. No 3. P. 8–11. [in Ukrainian]

Satarova T.M., Derkach K.V., Abraimova O.Ie. Otsinka retsyproknoho efektu v kul'turi in vitro u henotypiv kukurudzy zarodkovoi plazmy Lankaster. Biul. In-tu zernovoho hospodarstva. 2011. No 40. P. 20–24. [in Ukrainian]

Venkatramana P., Carlson C., Blackstad M., Bialozynski R., Schultz, Kaufman B. Development and characterization of single nucleotide polymorphism (SNP) panel for markers-assisted backcrossing in corn. Abstracts from AOSA/SCST Meeting. 2012. V. 32, No 2. P. 153.

Akinina G.E., Dugar Iu.N., Popov V.N. Statisticheskiy analiz geneticheskikh dannykh s ispol'zovaniem komp'iuternykh programm ARLEQUIN, PHILYP, CLANN, STRUCTURE. Khar'kov: Stil'-Izdat, 2014. 100 p. [in Russian]

Pritchard J.K., Stephens M., Donnelly P. Inference of population structure using multilocus genotype data. Genetics. 2000. V. 155. P. 945–959.