Отримання трансгенних рослин орхідеї Dendrobium linguella RCHB. F., що містять гени BAR та CYP11A1 цитохрому P450scc

Анотація

Aim. Dendrobium linguella is one of the most popular orchids sold as potted plants. This orchid has beautiful white-pink flowers. The aim of our work was to produce transgenic plants of D. linguella having better productivity and resistant to herbicide. Methods. Agrobacterium tumefaciens strain CV3101 carrying gene construct pCB093 was used for genetic transformation. Obtained calli were analyzed by PCR and RT-PCR. Results. 35 BASTA-resistant D. linguella lines were produced after Agrobacterium tumefaciens‑mediated transformation with a gene construct carrying bar, sgfp gene and cyp11A1 gene of cytochrome P450scc from bovine adrenal cortex. Integration of foreign genes in genome of all 17 plants analyzed was confirmed by PCR analysis. Transcription of cyp11A1 gene was shown by RT-PCR analysis. Conclusions. Transgenic plants of D. linguella resistant to commercial herbicides Basta, Bialaphos, Glufosinate were produced. In the future the expression of cyp11A1 gene and its influence on plant morphology will be studied.

Keywords: Dendrobium linguella, cyp11A1 gene, bar gene, PCR, herbicide.