Активність транспозона як чинник втрати функції гена Iw2(Т) у нащадків штучних амфідиплоідів Triticinae

Анотація

Aim. To check the possible role of retrotransposons in the variation initiation, which causes activity loss of Iw²(T) gene. Methods. REMAP–technic for detection changes in genetic sequences caused by transposon activity. Results. All hybrid lines which are considered to have an inactivated gene–inhibitor of glaucousness and are recessive mutants (Іw2(Т)→iw2(Т)) were characterized by appearance of additional component in the electrophoretic spectrum obtained with primers Xwms702F/RemapCAn. Conclusions. Possible molecular base of the mutation Іw2(Т)→iw2(Т) can be transposon movement, resulting in gene expression and release of a functional product.

Keywords: artificial wheat amphidiploids, glaucousness, Amblyopyrum muticum, REMAP, transposons.