Исследование полиморфизма длины интронов генов β-тубулина у растений рода Linum L.

Анотація

Aim. Fingerprinting with molecular markers allows to carry out precise and rapid species identification. Tubulin-Based-Polymorphism (TBP) was originally introduced as a novel method for assaying genetic diversity in plants. In this connection, testing of this method in genetic research of plants of the genus Linum L. is very promising. Methods. The CTAB-method for the isolation of DNA, PCR amplification with specific primers, electrophoretic analysis under non-denaturing polyacrylamide gel and staining by silver nitrate method were used. Results. Genetic polymorphism of flax species was described on the basis of TBP. It was found that the number of the amplicons that correspond the introns of β-tubulin genes can be varied from 400 bp to 1900 bp. The mean PIC value was about 0.3. Conclusions. Obtained data show that the intron length polymorphism method has a good ability to differentiate all studied species of flax. This information may be useful for the further genetic analysis of Linum.

Keywords: molecular markers, β-tubulin, Linum L., ТВР (tubulin base polymorphism).