Микросателлитная нестабильность в клетках линии лабораторных мышей ICR, предрасположенной к спонтанным новообразованиям

  • M. V. Kovalchuk Институт молекулярной биологии и генетики НАН Украины Украина, 03143, Киев, ул. Заболотного, 150
  • T. P. Gulko Институт молекулярной биологии и генетики НАН Украины Украина, 03143, Киев, ул. Акад. Заболотного 150; ГУ «Институт генетической и регенеративной медицины НАМНУ» Украина, 04114, м. Киев, ул. Вышгородская, 67

Анотація

Aims. Microsatellite instability (MSI) is a form of genomic instability frequently detected in many types of tumors. However, the involvement of MSI in cancer development in the population ICR mouse strain has not been investigated. The aim of this work was to determine the reveal of loss of heterozygosity(LOH) and microsatellite instability (MSI) and to evaluate the possibility ofusing LOH and MSI as molecular markers for the detection ofgenomic alterations. Methods. Genomic DNA obtained from peripheral blood lymphocytes from each animals was subjected to PCR using specific primers. The PCR products were separated by 10% non-denaturingPAGE and analyzed for the presence of LOH and MSI. Results. The presence of these microsatellitealterations was related both to the carcerous phenotype and to the mouse strain. Conclusion. This study has demonstrated that microsatellite alterations occurin a part of ICR population butour data need further validation with a larger number of microsatellite loci and enhanced method for MSI identification.

Key words: microsatellite instability, loss of heterozygosity, tumorigenesis.