Генетична трансформація in planta м’якої пшениці з використанням штаму AGLO, який містить pBi2E з дволанцюговим РНК-супресором гена проліндегідрогенази

Анотація

Aims. The effectiveness of (Triticum aestivum L.) transformation in planta using strain AGLO harboring plasmid pBi2E with dsRNA-suppressor (double sequence RNA-suppressor) of prolinedehydrogenase gene and selective neomycin phosphotransferase II gene (nptII) has been analyzed. Methods. Agrobacterium-mediated genes transfer in planta during wheat pollination. Results. Seeds, RCR-analysis of which confirmed availability pro1 gene exon, of T1- wheat’s plants have been obtained. Conclusions. It has been shown the possibility of stable integration of the transgenes at the wheat’s genome under Agrobacterium-mediated transformation in planta.

Keywords: Triticum aestivum, Agrobacterium-mediated transformation in planta.