Створення чужинно-заміщених та чужинно-доданих ліній Triticum aestivum / Aegilops mutica
Анотація
Aims. Development of T. aestivum / Ae. mutica introgressive lines and their assessment for powdery mildew resistance and morphological characters. Methods. Artificial hybridization, determination of chromosome numbers at mitosis metaphase, PCR, dot-blot hybridization of genome DNA. Results. AABBDT hybrid was sufficiently self-fertile; therefore, progeny derived from its self-pollination were used for the development of introgressive lines, according to chromosome mixing method. Avoidance of backcrossing to recurrent parent provided maximal retention of Aegilops chromosomes in progeny used for introgressive lines development. Conclusions. Derived introgressive lines are appropriate for the following identification of quantity and amount of alien introgressions and determination of their homoeological identity.
Key words: wheat introgressive lines, Ae. mutica, powdery mildew, frost resistance.