Однонуклеотидный полиморфизм гена интерлейкина-6 (IL-6) у жителей Харькова, больных сахарным диабетом 2 типа

Анотація

Aims. To examine the association between SNP rs2069840 (174G / C) of interleukin-6 gene and type 2 diabetes in the population of Kharkov. Objects were venous blood samples of 60 patients with type 2 diabetes and 49 healthy persons (all residents of Kharkiv, Ukrainian and Russian). Methods. Genotyping by PCR-RFLP. Results. The distribution of genotypes for SNP rs2069840 corresponds to the Hardy-Weinberg equilibrium. Allele frequencies in the control group is 0.5; in the group of patients p_C = 0.4, q_G = 0.6. The weak non-significant association between CC genotype and type 2 diabetes was marked. The odds ratio for genotype GG is 0.54. The sensitivity of the test is 15 %, specificity – 76 %. The positive predictive value is equal to 2 %, negative – 97 %, the accuracy of the test is 74 %, positive likelihood ratio result is 0.63, a negative – 1.6. Conclusions. The distribution of genotypes for SNP rs2069840 of interleukin-6 in the Kharkov population does not deviate from the Hardy-Weinberg equilibrium. According to the results of a pilot study was founded that the CC genotype increases the risk of type 2 diabetes in 1.2 times.

Keywords: type 2 diabetes mellitus, interleukin-6 gene, Kharkov population, allele frequencies.