Callus induction from shoot apical meristem in Triticum spelta L. and T. aestivum L.

  • A. V. Kyriienko
  • M. F. Parii
  • Yu. V. Symonenko
  • M. V. Kuchuk
  • N. L. Shcherbak
DOI: https://doi.org/10.7124/FEEO.v25.1169

Abstract

Aim. To develop an effective protocol for callus induction from shoot apical meristem in Triticum spelta L. and T. aestivum L. Methods. Plant material: spelt “Europe” and common wheat “Bunchuk”. For this research we used shoot apical meristems from 3-days plants. For callus induction we proposed 4 media with different concentration of 2,4-D, picloram, NAA and AgNO3. Explants were growing in dark during 21 day at + 25 C. Results. Calli were transparent and mild, less than 8 mm. For callus induction positive effect were shown on media with 2,4-D and picloram. At the same time, NAA was not such effective. Conclusions. In our research was shown, that the best media for spelt callus induction should have 2 mg/l 2,4-D and 10 mg/l AgNO3.

Keywords: callusogenesis, spelt (Triticum spelta L.), common wheat, callus, shoot apical meristem.

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