Transcriptional factor Cat8 is involved in regulation of xylose fermentation in engineered Saccharomyces cerevisiae

  • L. S. Dzanaieva
  • K. V. Dmytruk
  • A. A. Sybirny
DOI: https://doi.org/10.7124/FEEO.v22.970

Abstract

Aim. The aim of this work is the construction of cat8Δ strain on the base of xylose-fermenting S. cerevisiae strain and evaluation of the xylose fermentation rate. Methods. The CAT8 deletion cassette harboring natNT2 gene flanking with 5’ and 3’ non-coding regions of CAT8 gene has been constructed. After transformation by the cassette the cat8Δ strain was selected on the nourseothricin containing medium. Xylose fermentation experiments of constructed strain was performed in mineral medium supplemented with xylose under oxygen-limited conditions. Results. Xylose-fermenting cat8Δ S. cerevisiae strain has been constructed by homologous recombination of the CAT8 deletion cassette with target sequences in the genome of GS010 strain. The cat8Δ strain possessed increase in ethanol accumulation, ethanol yield, rate of ethanol production and productivity of ethanol synthesis relative to the parental GS010 strain for 9.5, 6, 20 and 12 %, respectively. Conclusions. The mutant of the xylose-fermenting S. cerevisiae strain with knock out of the CAT8 gene coding for transcriptional activator, has been constructed. The cat8Δ mutant showed 9.5 % increase in ethanol production from xylose relative to parental strain.

Keywords: alcoholic fermentation, xylose, S. cerevisiae, Cat8.

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