Optimization of conteining dermal coverages production for burn wound treatment at in vivo model

  • L. L. Matsevych
  • A. Ye. Papuga
  • T. P. Ruban
  • T. V. Beregova
  • L. L. Lukash
DOI: https://doi.org/10.7124/FEEO.v22.963

Abstract

Aim. The aim was to estimate the influence of cell suspension quality on the therapeutic efficiency of cell-containing dermal coverages in animal model in vivo. Methods. We carried out the application of gel wound coverages with different quality of cellular compound on the third degree burns of ICR line mice. In the negative control group animals were treated with fresh medium-containing gel, and in positive control – by gel containing high quality cell suspension. Photo fixation of burn wound status was carried out once a day. The results were estimated by ANOVA approach. Results. There was a statistically significant difference of burn wounds development and healing between positive control and both experimental groups. The decreasing of alive cell fraction in prepared gel within 5 hours has been shown as well. Conclusions. It has been shown the dependence of wound healing properties of coatings containing cells on the cell compound quality, in particular, from the viability and integrity of cells. These results are important for developing of clinical protocols using such cell‑containing dermal equivalents.

Keywords: burn wound, dermal equivalent, stem cells, skin substitute, tissue engineering.

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