DNA certification of varieties and hybrids of perennial grass crops

  • I. P. Kondratskaya
  • A. N. Yukhimuk
  • O. V. Chizhik
  • V. N. Reshetnikov
  • V. A. Stolepchenko
  • P. P. Vasko
DOI: https://doi.org/10.7124/FEEO.v22.960

Abstract

Aim. The aim of this study was to carry out passportization of cultivars, varieties and hybrids of perennial grass crops: Alopecurus protensis, Agropyron cristatum, Lolium perenne, Lolium miltiflorum, Festuca protensis, Festuca arundinasea, hybrids Festulolium. Methods. The perennial grasses selection was carried out by embryo culture according to methodical recommendations. For the molecular-genetic passportization of perennial grasses varieties the polymorphous primers have been selected – 9 RAPD primers and 15 ISSR primers. Results. Multilocus DNA labeling have allowed to differentiate all the genotypes being studied, to develop and to create the unique profiles for each of them and to calculate the genetic distance of congeniality/distances. On the base of RAPD/ISSR, SCoT spectra the 14 molecular-genetic passports of perennial grass cops have been obtained for the first time.

Keywords: grass crops, molecular-genetic passport, DNA, PCR, multipoint mapping RAPD/ISSR spectra.

References

The law of the Republic of Belarus of 13.04.1995 № 3725-XII "On patents on plant varieties" (edited by the Law of the Republic of Belarus of 17 may 2011 No. 266-3).

Methodical instructions on selection of perennial grasses. Moskva, 1985. 188 p.

Dempster E.L., Pryor K.V., Francis D., Young J.E., Rogers H.J. Rapid DNA extraction from ferns for PCR-based analyses. Biotechniques. 1999. Vol. 27 (1). P. 66–68. doi: 10.2144/99271bm13

Collard B.C.Y., Mackill D.J. Start codon targeted (SCoT) polymorphism: a simple, novel DNA marker technique for generating gene-targeted markers in plants. Plant Mol Biol Rep. 2009. Vol. 27. P. 86–93. doi: 10.1007/s11105-008-0060-5

Mirzaei K., Mirzaghaderi G. Genetic diversity analysis of Iranian Nigella sativa L. landraces using SCoT markers and evaluation of adjusted polymorphism information content. Plant Genetic Resources: Characterization and Utilization. 2005. P. 1–8. doi: 10.1017/S1479262115000386

Paäakinskiene I., Griffiths C.M., Bettany A.J.E., Paplauskiene V., Humphreys M.W. Anchored simple-sequence repeats as primers to generate speciesspecific DNA markers in Lolium and Festuca grasses. Theor Appl Genet. 2000. Vol. 100. P. 384–390. doi: 10.1007/s001220050050

Arghavani A., Asgharu A., Shokrpour M., Chmanabad M. Genetic diversity in ecotypes of two Agropyron species using RAPD markers. Research Journal of Environmental Sciences. 2010. Vol. 4 (1). P. 50–56. doi: 10.3923/rjes.2010.50.56