Genotypic features of morphogenetic reactions of varieties and hybrids F1 of winter soft wheat during various stages of androgenesis in vitro

  • I. S. Zambriborshch
  • O. L. Shestopal
  • M. S. Boyko
DOI: https://doi.org/10.7124/FEEO.v22.957

Abstract

Aim. Testing the haploproduction ability of 47 varieties and 143 F1 hybrids of winter bread wheat. Methods. In vitro culture of isolated anthers of wheat. The percentage of callus and regeneration of green plants for each genotype calculated as a percentage of the planted anthers. Results. The differences in the frequency of сallus induction and the ability to regenerate plants in the process of androgenesiss in vitro of winter soft wheat were detected. The range of variation haploproduction activiti was broad. The sign of "the formation of сallus" for varieties was in limited from 0 to 21.2 % and for hybrids – from 0 to 38.8 %; on the sign of "regeneration of green plants" for varieties – from 0 to 9.4 %, and for hybrids from 0 to 9.1 %. The positive effect of 1BL/1RS translocation on signs of haploproduction in anther culture Triticum aestivum L. Conclusions. Genotype-specific of microspores morphogenetic reactions of soft winter wheat in the process of androgenesis in vitro were revealed. It has been found that F1 hybrids have a higher potential production potential in anther culture than varieties. During the period from 2011 to 2017, the 580 lines of winter wheat were transferred to the breeding departments.

Keywords: soft winter wheat, anther culture in vitro, callus, regeneration.

References

Diyachuk T.Y., Stolyarova S.V., Khomyakova O.V., Koldyreva A.V., Ytaliyanskaya Gu.V., Safronova N.F., Medvedeva L.P. The role of the haploidy method in creating the initial material for the selection of cereals in the NIISH of the South-East. Genom of plants. Odesa, 2008. Р. 178–181.

Mihaly R., Lantos C., Kertesz Z., Mesterhazy A., Pauk J. Using of doubled haploids in wheat breeding. Haploid in Higher Plants: III. Intern. Conf (Vienna, February 12–15, 2006). P. 34.

Litvynenko M.A. Biotechnological methods in selection of agricultural crops. Bulletin of Agrarian Science.. 2010. No. 6. P. 11–14.

Ignatova S.A. Biotechnological foundations for obtaining haploids, distant hybrids and somatic regenerants of cereals and legumes in various invitro systems: author’s abstract. dis. ... Dr. Biol. sciences. Yalta, 2004. 45p.

Ignatova S.O., Zhosonar M.V., Lobanova K.I., Shestopal O.L. Getting haployidiv doubling in wheat anther culture: Methodical recommendations. Odesa, 2008. 12 p.

Lobanova K.I. Regeneration plants in anther culture of wheat, ways to increase AI: author’s abstract. dis. ... Candidate biol. sciences Odessa, 2009. 19 p.

Atramentova L.O., Utjevskaya O.M. Statistical methods in biology. Kh: KhNU named V.N. Karazina, 2007. 288 p.

Zhosonar M.V., Ignatova S.A., Vait V.I., Fedorova V.R. Regeneration capacity of different vernalization and photoperiodic sensitivity of winter wheat varieties in anther culture in vitro. Bulletin of Kharkiv National Agrarian University. Series: biology. Kharkiv, 2004. Vol. 2 (5). P. 79–83.

Lobanova E.I., Ignatova S.A., Shestopal O.L., Narhan T.P. Regeneration in anther culture of winter wheat genotypes from different lengths of time "shoots-earing". Factor of Experimental Evolutionary Organisms. 2008. Vol. 5(5). P. 291–297.

Ignatova S.O., Zhosonar M.V., Lobanova K.I. Features identify the level of sensitivity to androgenic different genotypes of soft wheat anther culture. Physiology and biochemistry of cultivated plants. 2010. Vol. 42, No. 2 (244). P. 107–117.

Shestopal O.L., Zambriborshch I.S., Topal M.M., Lytvynenko M.A., Ignatova S.O. The study of the haploproduction ability of soft wheat with wheat-rye translocations. Factors of experimental evolution organisms. 2013. Vol. 12. P. 326–330.

Shestopal O.L., Zambriborshch I.S., Topal M.M. Haploproduction capacity of soft winter wheat from the presence of wheat-rye translocations in the genotype. Bulletin of Kharkiv National Agrarian University. Series: biology. Kharkiv, 2014. Vol. 23. P. 51–56.

Ignatova S.O., Lobanova K.I., Shestopal O.L. Method of forecasting the level of plant regeneration in the culture of winter wheat pests: Pat. to utility model 36253 Ukraine. No. u 200803034; stated. 11.03.08, published. 27.10.08, Bull. No. 20.

Ignatova S.O., Lobanova K.I., Shestopal O.L. Method of increasing the capacity of regeneration in winter wheat anther culture: Pat. to the utility model 21988 Ukraine. No. u 200611658; stated. 6.11.06, pub. 10.04.07, Bul. No. 4.

Henry Y., Bernard S., Bernard M., Gay G., Marcotte J.-L., De Buyser J. Nuclear gametophytic genes from chromosome arm 1RS improve regeneration of wheat microspore-derived embryos. Genome. 1993. Vol. 36(5). P. 808–814. doi: 10.1139/g93-106

Henry Y., de Buyser J. Effect of the 1B/1R translocation on anther culture ability in wheat (Triticum aestivum L.). Plant Cell Reports. 1985. Vol. 4 (6). P. 307–310. doi: 10.1007/BF00269885