Genotyping of Ukrainian common wheat cultivars using the marker of the Lr48 gene conferring moderate resistance to leaf rust

  • A. V. Karelov
  • N. A. Kozub
  • I. A. Sozinov
DOI: https://doi.org/10.7124/FEEO.v22.929

Abstract

Aim. Common wheat (Triticum aestivum L.) is one of the most important and widely cultivated crops over the world. For a lot of wheat diseases introduction of resistance genes is considered to be the most rational way to diminish yield losses and control spread of causal agents. The aim of this research was to study a sample of Ukrainian common wheat cultivars with the use of the molecular genetic marker for the Lr48 gene. Methods. DNA samples of 46 common wheat cultivars developed in the Remeslo Myronivka Institute of Wheat (RMIW) of National Academy of Agrarian Sciences jointly with the Institute of Plant Physiology and Genetics of National Academy of Sciences of Ukraine were analyzed with the use of the marker IWB70147. Results. It was revealed that 15 out of 46 (or 32.6 %) cultivars carried resistance-associated allele of the marker. Conclusions. It was revealed that the resistance-associated allele of the marker of the Lr48 gene is present in Ukrainian common wheat cultivars developed in the Forrest Steppe zone of Ukraine. The possible source of the resistance allele is „Mironovskaya 808‟ which is in the pedigree of many Ukrainian and world wheat cultivars. The data obtained in this research can be used in breeding programs to select sources of moderate adult plant resistance. Cultivars „Yuviliar Myronivskii‟, „Volodarka‟ and „Pamyati Remesla‟ with adult leaf rust resistance conferred by the Lr48 gene also carry resistance associated allele of the Lr34/Yr18/Pm38/Sr57/Bdv1 gene.
Keywords: molecular markers, wheat, resistance genes, adult plant resistance.

References

Bushuk W., Rasper V.F. (eds.) Wheat: production properties and quality. Blackie/Chapman and Hall, London, 1994. 239 p.

Johnson R. Past, present and future opportunities in breeding for disease resistance, with examples from wheat. Euphytica. 1992. Vol. 63, No. 1–2. P 3–22.

Sharma I. (ed.) Disease Resistance in Wheat. Hardback, Punjab Agricultural University, India, 2012. 336 p.

Langridge P., Lagudah E.S., Holton T.A., Appels R., Sharp P.J., Chalmers K.J. Trends in genetic and genomic analyses in wheat: A Review. Australian J Agric Res. 2001. Vol. 52. P. 1043–1078.

Lesser W., Kolady D.E. Disease Resistance of Wheat Varieties: Can Private Varieties Withstand the Pressure? Economics Research International. 2011. Vol. 2011. 6 p. doi: 10.1155/2011/575192

Ellis J.G., Lagudah E.S., Spielmeyer W., Dodds P.N. The past, present and future of breeding rust resistant wheat. Frontiers in Plant Science. 2014. Vol. 5, Iss. 641. doi: 10.3389/fpls.2014.00641

Bolton M.D., Kolmer J.A., Garvin D.F. Wheat leaf rust caused by Puccinia triticina. Mol Plant Pathol. 2008. Vol. 9, No. 5. P. 563–575.

Gupta V.S., Khan R.R., Rajwade A.V., Reddy, D.M.R., Hosmani P., Dholakia B.B., Lagu M.D., Tamhankar S.A., Rao V.S., Sain, R.G. Molecular Mapping of Leaf Rust Resistance Gene Lr15 in Bread Wheat. 11th Int. Wheat Genet. Symp. Sydney: Univ. press, 2008. P. 724–726.

Cloutier S., McCallum B.D., Loutre C., Banks T.W., Wicker T., Feuillet C., Keller B., Jordan M.C. Leaf rust resistance gene Lr1, isolated from bread wheat (Triticum aestivum L.) is a member of the large psr567 gene family. Plant Mol Biol. 2007. Vol. 65, No. 1–2. P. 93–106.

Huang L., Brooks St.A, Li W., Fellers J.P., Trick H.N., Gill B.S. Map-based cloning of leaf rust resistance gene Lr21 from the large and polyploid genome of bread wheat. Genetics. 2003. Vol. 164, No. 2. P. 655–664.

Kolmer J.A. Genetics of resistance to wheat leaf rust. Annu Rev. Phytopathol. 1996. Vol. 34. P. 435–455.

Boskovic J., Boskovic M., Babovic M., Jerkovic Z., Pesic V. Pyramiding Strategy for Durable Resistance to Wheat Leaf Rust Pathogen. Wheat in a Global Environment. Developments in Plant Breeding / Bedö Z., Láng L. (eds). 2001. Vol. 9. P. 337–343.

Collard B.C., Mackill D.J. Marker-assisted selection: an approach for precision plant breeding in the twenty-first century. Philosophical Transactions of the Royal Society B: Biological Sciences. 2008. Vol. 363 (1491). P. 557–572.

Karelov A.V., Pirko Ya.V., Kozub N.A., Sozinov I.A., Pirko N.N., Litvinenko N.A., Lyfenko S.F., Koliuchii V.T., Blume Ya.B., Sozinov A.A. Identification of the allelic state of the Lr34 leaf rust resistance gene in soft winter wheat cultivars developed in Ukraine. Cytology and Genetics. 2011. Vol. 45, No. 5. P. 3–10.

Karelov A.V., Kozub N.A., Sozinov I.A., Sozinov A.A., Blume Ya.B. Allelic state of the molecular genetic markers for genes associated with sensitivity to Pyrenophora tritici-repentis toxins A and B and Staganospora nodorum toxin A among Ukrainian common wheat cultivars. The Bulletin of Vavylov Society of Geneticists and Breeders of Ukraine. 2015. Vol. 13, No. 1. P. 11–17.

Karelov A.V., Kozub N.A., Sozinov I.A., Borzykh A.I. Polymorphism of the TDF_076_2D gene conferring moderate Fusarium head blight resistance among common wheat (Triticum aestivum L.) cultivars of the steppe zone of Ukraine. Scientific reports of NULES of Ukraine. 2015. No. 2 (51). URL: http://nd.nubip.edu.ua/2015_2/7e.pdf (Last accessed: 02.28.2018).

Nsabiyera V., Qureshi N., Bariana H.S., Wong D., Forrest K.L., Hayden M.J. Molecular markers for adult plant leaf rust resistance gene Lr48 in wheat. Molecular Breeding. 2016. Vol. 36, No. 65. doi: 10.1007/s11032-016-0488-5.

Saini R.G. Kaur M., Singh B., Sharma S., Nanda G.S., Nayar S.K, Gupta A.K., Nagarajan S. Genes Lr48 and Lr49 for hypersensitive adult plant leaf rust resistance in wheat (Triticum aestivum L.). Euphytica. 2002. Vol. 124. P. 365–370.

Bansal U.K., Hayden M.J., Venkata B.P., Khanna R., Saini R.G., Bariana H.S. Genetic mapping of adult plant leaf rust resistance genes Lr48 and Lr49 in common wheat. Theor Appl Genet. 2008. Vol. 117, No. 3. P. 307–312.

Singh A., Pallavi J.K., Gupta P., Prabhu K.V. Identification of microsatellite markers linked to leaf rust adult plant resistance (APR) gene Lr48 in wheat. Plant Breeding. 2011. Vol. 130, No. 1. P. 31–34.

GRIS: Genetic Resources Information System for Wheat and Triticale (database). URL: http://www.wheatpedigree.net/ (Last accessed: 02.28.2018).