Bystander effect formation in the sprout root meristem in condition of irradiated and non-irradiated seeds joint growing

  • O. Yu. German
DOI: https://doi.org/10.7124/FEEO.v22.919

Abstract

Aim. To study the possibility of bystander effect inducing in the joint germination in a common aqueous environment of irradiated and non-irradiated seeds. Methods. Cytogenetic methods including mitotic activity level estimation, accounting for the number of nucleoli, and methods for statistical analysis of the data. Results. The effect of radiation stimulation with a dose of 40 Gy was dependent on the variety, and the effect of gamma radiation at a dose of 200 Gy reduced the level of mitotic activity in comparison with the control in both varieties. Mitotic indices increase in the meristem of non-irradiated seeds while growing with irradiated one. Conclusions. The bystander effect is formed on the organismic level under condition of growing of irradiated and non-irradiated seeds in a common aquatic environment and it is ma-nifested as an increase in the level of meristem cells proliferation and in the intensification of synthetic processes. The addition of irradiated with a high dose of gamma radiation seeds to the non-irradiated seeds induces an increase in mitotic activity largely than the addition of seeds irradiated with a relatively small dose.

Keywords: bystander effect, radiation stimulation, mitotic activity, nucleoli.

References

Widela M., Lalika A., Krzywona A., Poleszczuk J., Fujarewicz K., Rzeszowska-Wolny J. The different radiation response and radiation-induced bystander effects in colorectal carcinoma cells differing in p53 status. Mut. Res. Fund. Mol. Mech. Mutagen. 2015. Vol. 778. P. 61–70. doi: 10.1016/j.mrfmmm.2015.06.003.

Ermakov A.V., Kostyuk S.V., Egolina N.A., Malinovskaya E.M., Veyko N.N., Spitkovskiy D.M. DNA fragments found in the culture medium after exposure to ionizing radiation in adapting doses are a means of stress signaling between lymphocytes and the witness cells. Radiatsionnaya biologiya. Radioekologiya. 2007. Vol. 47, N. 2. P. 133–140.

Reis P., Lourenço J., Carvalho F.P., Oliveira J., Malta M., Mendo S., Pereira R. RIBE at an inter-organismic level: a study on genotoxic effects in Daphnia magna exposed to waterborne uranium and a uranium mine effluent. Aquatic Toxicology. 2018. doi: 10.1016/j.aquatox.2018.03.007.

Vorobtsova I.E., Kolesnikova I.S. Investigation of Radiation-Induced “Bystaner Effect” Using Model of Adaptive Response in Mixed Lymphocyte Culture from Humans of Different Gender. Radiatsionnaya biologiya. Radioekologiya. 2007. Vol. 47, N. 6. P. 1–5.

Verma N., Tiku A.B. Significance and nature of bystander responses induced by various agents. Mutation Research/Reviews in Mutation Research. 2017. Vol. 773. P. 104–121. doi: 10.1016/j.mrrev.2017.05.003.

Shemetun O.V., Pilinska M.A. Adiation-induced bystander effect. Tsitologiya i Genetika. 2007. Vol. 41, N. 4. P. 66–71.

German O.Yu., Dolgova T.A. Researches on plant cytophysiology and radiation genetics at the biological station of V.N. Karazin Kharkov National University. The Journal of V.N. Karazin Kharkiv National University. Series: biology. 2014. Vol. 20, N. 1100. P. 22–28.

Atramentova L.O., Utevska O.M. Statistical methods in biology. Kharkiv: V.N. Karazin Kharkov National University, 2007. 288 p.

Mothersill C., Seymour C. Relevance of radiation-induced bystander effects for environmental risk assessment. Radiatsionnaya biologiya. Radioekologiya. 2002. Vol. 42, N. 6. P. 586–587.

Kravets A.P., Vengzhen G.S., Grodzinskiy D.M. Effects of remote interaction of irradiated and non-irradiated plants. Radiatsionnaya biologiya. Radioekologiya. 2009. Vol. 49, N. 4. P. 490–494.

Koterov A.N. Prospects for the registration of the "bystander effect" in the assessment of radiation risks. Mediko-biologicheskie problemyi zhiznedeyatelnosti. 2011. N. 1 (5). P. 7–19.

Koterov A.N., Vaynson A.A. Biological and medical effects of low LET radiation for different dose ranges. Meditsinskaya radiologiya i radiatsionnaya bezopasnost. 2015. Vol. 60, N. 3. P. 5–31.

Littl D.B. Non-existent effects of ionizing radiation: conclusions regarding low-dose exposures. Radiatsionnaya biologiya. Radioekologiya. 2007. Vol. 47, N. 3. P. 262–272.