Deschampsia antarctica E. Desv. plants with different chromosome number cultivated in vitro. Plants length and flavonoids in in vitro culture and in nature

  • O. O. Poronnik Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Ukraine, 03680, Kyiv, Akad. Zabolotnogo str., 150
  • I. Yu. Parnikoza Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Ukraine, 03680, Kyiv, Akad. Zabolotnogo str., 150
  • N. Yu. Miryuta Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Ukraine, 03680, Kyiv, Akad. Zabolotnogo str., 150
  • G. Yu. Myryuta Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Ukraine, 03680, Kyiv, Akad. Zabolotnogo str., 150
  • V. P. Grakhov M.M. Gryshko National Botanical Garden, Ukraine, 01014, Kyiv, Tymeryazivska str., 1
  • D. O. Navrotska Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Ukraine, 03680, Kyiv, Akad. Zabolotnogo str., 150
  • V. A. Kunakh Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Ukraine, 03680, Kyiv, Akad. Zabolotnogo str., 150
DOI: https://doi.org/10.7124/FEEO.v20.785

Abstract

Aim. The aim of this work is to research two adaptability indices (leaf length and flavonoid content and spectrum) in eleven genotypes revealed in seeds germ of Deschampsia antarctica E. Desv. in standardized cultivation condition in vitro. Then these adaptability indices would compare with the same for native populations. Methods. We have used biochemical method for determination of flavonoid contents in plant leaves, HPLC method for research of flavonoid spectrum in plant leaves. Results. Heterogeneity of leaf length and flavonoid content has been shown. Both parameters difference have been detected in comparison with native populations. Flavonoid spectrum has no difference in comparison with native populations. Conclusions. Deschampsia antarctica genotypes under standardized cultivation condition in vitro have had longer leaves than plants in nature populations. Length of the leaves was varied depending on karyotype of corresponding genotype. Cultivated plants have had flavonoid spectrum similar to the same plants in nature. This fact allows to assume plants in vitro possible producent of secondary metabolites.

Keywords: Deschampsia antarctica, leaf length, flavonoids, chromosome number.

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