Deschampsia antarctica E. Desv. plants with different chromosome number cultivated in vitro. Probabilistic relations of three adaptability indices with genome size

  • N. Yu. Miryuta Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Ukraine, 03680, Kyiv, Zabolotnogo str., 150
  • I. Yu. Parnikoza Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Ukraine, 03680, Kyiv, Zabolotnogo str., 150
  • O. O. Poronnik Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Ukraine, 03680, Kyiv, Zabolotnogo str., 150
  • G. Yu. Myryuta Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Ukraine, 03680, Kyiv, Zabolotnogo str., 150
  • V. A. Kunakh Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Ukraine, 03680, Kyiv, Zabolotnogo str., 150

Abstract

Aim. This work deals with probabilistic relations between genome size and adaptability indices research in 11 genotypes plants revealed among seeds of Deschampsia antarctica E. Desv. which has different genome size under standardized cultivation condition in vitro. Methods. We have used electrophoretic method for determination of protein relative contents in plant leaves, and statistical analysis for probabilistic relations determination. Results. Protein densitometric profiles for 11 D. antarctica genotypes plants were analyzed. Probabilistic scheme have been created for all researched plants genotypes for all previously analyzed parameters. Three probabilistic schemes of genotypes plants received from seeds collected in the same loci and season have been shown and analyzed. Conclusions. Protective protein densitometric profiles were similar for 11 genotypes of D. antarctica plants under standardized cultivation condition in vitro but quantitative part of protective protein has been changed. Genome size had an influence on its probabilistic relations with flavonoids content, leaf length and some protein relative contents in general pool. Probabilistic relations between genome size and some protein relative content and between the last and flavonoids content and leaf length is assumed to express underlying in this plants adaptation process to uniqueness nature condition in dynamic hereditary memory special information in certain degree. Mechanisms of probabilistic relations changes are required a detailed research.
Keywords: Deschampsia antarctica, plants in vitro, probabilistic relations between genome size and adaptability indices, plant adaptability.

References

Lee J., Noh E.K., Choi H.S., Shin S.C., Park H., Lee H. Transcriptome sequencing of the Antarctic vascular plant Deschampsia antarctica Desv. under abiotic stress. Planta. 2012. doi: 10.1007/s00425-012-1797-5.

Parnikoza I., Kozeretska I., Kunakh V. Vascular Plants of the Maritime Antarctic: Origin and Adaptation. American Journal of Plant Sciences. 2011. 2. P. 381–395.

Andreev I.O., Spiridonova E.V., Kyryachenko S.S., Parnikoza I.Yu., Maidanyuk D.N., Volkov R.A., Kozeretska I.A., Kunakh V.A. Population-genetic analysis of Deschampsia antarctica from two regions of maritime Antactic. Moscow University Biological Sciences Bulletin. 2010. V. 65(4). P. 208–210.

Churaev R.N. Epigenetika: gennye i epigennye seti v onto- i filogeneze. Genetika. 2006. V. 42(9). P. 1276–1296. [in Russian]

Metody biokhimicheskogo issledovaniia rasteniy / Pod red. A.I. Ermakova. Leningrad, 1987. 191 p. [in Russian]

Pobezhimova T.P., Kolesnichenko A.V., Grabitel'ny O.I. Metody izucheniia mitokhondriy rasteniy. Poliarografiia i elektroforez. Moskva: Nauka, 2004. 98 p. [in Russian]

Scion Image. Retrieved from: http://scion-image.software.informer.com/4.0/.

Pollard Dzh. Spravochnik po vychislitel'nym metodam statistiki. Moskva: Finansy i statistika. 1982. 344 p. [in Russian]

Ayvazian S.A., Bukhshtaber V.M., Eniukov I.S., Meshalkin L.D. Prikladnaia statistika. Klassifikatsiia i snizhenie razmernosti. Moskva: Finansy i statistika, 1989. 606 p. [in Russian]

Parnikoza I., Miryuta N., Ozheredova I., Kozeretska I., Smykla J., Kunakh V., Convey P. Comparative analysis of Deschampsia antarctica Desv. population adaptability in the natural environment of the Admiralty Bay region (King George Island, maritime Antarctic). Polar Biology. 2015. V. 38(9). P. 1401–1411.

Miriuta N., Poronnik O., Parnikoza I., Hrakhov V., Myriuta H., Kozub N., Sozynov I., Kunakh V. Zberezhennia unikal'nosti za kompleksnoiu prystosovuvanistiu riznykh henotypiv Deschampsia antarctica Desv. v umovakh standartyzovanoho vyroshchuvannia roslyn in vitro. Ukrains'kyy Antarktychnyy Zhurnal. 2016. No. 15. P. 60–80. [in Ukrainian]

Sozinov A.A. Polimorfizm belkov i ego znachenie v genetike i selektsii. Moskva: Nauka, 1985. 272 p. [in Russian]