Lead and cadmium influences on the metallothionein expression level in Lymnaea stagnalis L. adults

  • S. E. Dromashko Institute of Genetics and Cytology of the National Academy of Sciences of Belarus, Belarus, 220072, Minsk, Akademicheskaya str., 27
  • S. N. Shevtsova Institute of Genetics and Cytology of the National Academy of Sciences of Belarus, Belarus, 220072, Minsk, Akademicheskaya str., 27
  • A. S. Babenko N.N. Aleksandrov Republican Scientific and Practical Centre for Oncology and Medical Radiology, Belarus, 223040, Minsk region, Lesnoy town
DOI: https://doi.org/10.7124/FEEO.v20.780

Abstract

Aim. The article deals with the assessment of some heavy metals effects on metallothionein expression in such a test system as the great pond snail L. stagnalis. Methods. There are methods of mollusk specimen cultivation and gene expression level estimation (PCR in real-time mode), as well as data analysis. Results. A significant increase in MT gene expression was observed in both young (4.5 months) and older (7 months) mollusks on the 7th day of exposure to 0.003 mg/L of lead ions as well as significant increase in MT gene expression was observed in older mollusks on the 7th day of exposure to 0.0001–0.001 mg/L of cadmium ions. Conclusions. The expediency of L. stagnalis adults using at the age of 3 to 5 months with other test systems for biotesting liquid metal-containing waste based on the gene expression evaluation in a short (no more than 7 day) experiment using the Real Time PCR has been shown.
Keywords: Lymnaea stagnalis, lead/cadmium, metallothioneins, gene expression, real time PCR.

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