Lifespan in adults of Drosophila melanogaster Meig. after exposure to microwave irradiation
Abstract
Aim. The purpose of investigation was to study the effect of microwave irradiation of varying intensity to lifespan in adults of Drosophila melanogaster Meig. Methods. Experiments were carried out on wild type Oregon-R strain. Two-day-old flies were irradiated by microwaves (frequency – 36.64 GHz, exposure time – 10 sec). The power density was 0.01, 0.1 and 1 W/m2. Lifespan was examined in the irradiated and non-irradiated (control) flies. Results. The females showed a reduction in life expectancy by power density of 0.01 and 1 W/m2, median decreased respectively by 6.0 % and 8.7 % (p < 0.05). In males, reduced life expectancy was found by microwave power density of 1 W/m2, the median fell by 11.8 % (p < 0.05). No effect was detected by irradiation intensity of 0.1 W/m2. Conclusions. Exposure to microwave irradiation can reduce the lifespan in Drosophila adults. The effect depends on the sex and power density of microwaves.
Keywords: Drosophila melanogaster, lifespan, non-ionising radiations.
References
Shckorbatov Y. The main approaches of studying the mechanisms of action of artificial electromagnetic fields on cell. Journal of Electrical & Electronic Systems. 2014. V. 3 (2). Р. 123. doi: 10.4172/2332-0796.1000123.
WHO/International Agency for Research on Cancer (IARC). Press Release. 2011. No. 208, 31 May.
WHO International EMF Project [Electronic resource]. 1997. Retrieved from: www.who.int/entity/peh-emf/en.
Medawar P.B. Old age and natural death. Modern Quarterly. 1946. V. 2. P. 30–49.
Vilenchik M.M. Molekuliarnye mekhanizmy stareniia. Moskva: Nauka, 1970. 168 p. [in Russian]
Harman D. Origin and evolution of the free radical theory of aging: a brief personal history, 1954–2009. Biogerontology. 2009. V. 10. P. 773–781. doi: 10.1007/s10522-009-9234-2
Williams G.C. Pleiotropy, natural selection and the evolution of senescence. Evolution. 1957. V. 11. P. 398–411. doi: 10.1111/j.1558-5646.1957.tb02911.x
Skulachev V.P. Starenie organizma – osobaia biologicheskaia funktsiia, a ne rezul'tat polomki slozhnoy zhivoy sistemy: biokhimicheskoe obosnovanie kontseptsii Veysmana. Biokhimiia. 1997. V. 62. P. 1369–1399. [in Russian]
Linnane A.W., Marzuki S., Ozawa T., Tanaka M. Mitochondrial DNA mutations as an important contributor to ageing and degenerative diseases. Lancet. 1989. V. 1. P. 642–645. doi: 10.1016/S0140-6736(89)92145-4
Vaiserman A.M. Transgenerational Inheritance of Longevity: An Epigenetic Phenomenon? J. Gerontol. Geriat. Res. 2012. V. 1(4). doi: 10.4172/2167-7182.1000e116.
Moskalev A., Aliper A., Smit-McBride Z., Buzdin A., Zhavoronkov A. Genetics and epigenetics of aging and longevity. Cell Cycle. 2014. V. 13(7). Р. 1063–1077. doi: 10.4161/cc.28433
Krut'ko V.N., Slavin M.B., Smirnova T.M. Matematicheskie osnovaniia gerontologii. Moskva: Editorial URSS, 2002. 384 p. [in Russian]
Glants S. Mediko-biologicheskaia statistika. Moskva: Praktika, 1998. 459 p. [in Russian]
Shckorbatov Y., Pasiuga V., Shakina L., Grabina V., Kolchigin N., Ivanchenko D., Kazansky O., Bykov V. Drosophila melanogaster viability and mutability under the influence of low energy microwave monochromatic and ultra wideband impulse field. 6-th International Conference on Antenna Theory and Techniques (17–21 September 2007, Sevastopol, Ukraine). 2007. Р. 289–291. doi: 10.1109/ICATT.2007.4425188
Atli E., Unlu H. The effects of microwave frequency electromagnetic fields on the development of Drosophila melanogaster. Int. Journ. Radiat. Biol. 2006. V. 82. P. 435–441. doi: 10.1080/09553000600798849
Atli E., Unlu H. The effects of microwave frequency electromagnetic fields on the fecundity of Drosophila melanogaster. Turk. Journ. Biol. 2007. V. 31. P. 1–5.
Zaliubovskaia N.P. Reaktsiia zhivykh organizmov na deystvie elektromagnitnykh millimetrovykh voln. Uspekhi fizicheskikh nauk. 1973. V. 110. P. 462–464. [in Russian]
Weisbrot D., Lin H., Ye L., Blank M., Goodan R. Effects of mobile phone radiation on reproduction and development in Drosophila melanogaster. Journal of Cell Biochemistry. 2003. V. 89. Р. 48–55. doi: 10.1002/jcb.10480
Panagopoulos D.J., Chavdoula E.D., Nezis I.P., Margaritis L.H. Cell death induced by GSM 900-MHz and DCS 1800-MHz mobile telephony radiation. Mutation Research. 2007. V. 626. Р. 69–78. doi: 10.1016/j.mrgentox.2006.08.008
Marec F., Ondracek J., Brunnhofer V. The effect of repeated microwave irradiation on the frequency of sex-linked recessive lethal mutations in Drosophila melanogaster. Mutation Research. 1985. V. 157. Р. 163–167. doi: 10.1016/0165-1218(85)90112-0
Hamnerius Y., Rasmuson A., Rasmuson B. Biological effects of high frequency electromagnetic fields on Salmonella typhimurium and Drosophila melanogaster. Bioelectromagnetics. 1985. V. 6. P. 405–414. doi: 10.1002/bem.2250060407
Dyka L.D., Strashniuk V.Yu., Shkorbatov Yu.H. Komponenty prystosovanosti u Drosophila melanogaster za vplyvu mikrokhvyl'ovoho oprominiuvannia. Visnyk Kharkivs'koho nats. un-tu imeni V. N. Karazina. Seriia «Biolohiia». 2016. No. 26. P. 65–73. [in Ukrainian]
Shckorbatov Y.G., Pasiuga V.N., Kolchigin N.N., Grabina V.A., Ivanchenko D.D., Bykov V.I., Dumin O.M. Cell nucleus and membrane recovery after exposure to microwaves. Proceedings of the Latvian Academy of Sciences. 2011. Section B 65 (672/673):13–20. doi: 10.2478/v10046-011-0013-5
Ennamany R., Fitoussi R., Vie K., Rambert J., Benetti L.D., Mossalayi M.D. Exposure to electromagnetic radiation induces characteristic stress response in human epidermis. Journal of Investigative Dermatology. 2008. V. 128. Р. 743–746. doi: 10.1038/sj.jid.5701052.
Vijayalaxmi, Cao Y., Scarfi M.R. Adaptive response in mammalian cells exposed to non-ionizing radiofrequency fields: A review and gaps in knowledge. Mutation Research – Review in Mutation Research. 2014. V. 270. P. 36–45. doi: 10.1016/j.mrrev.2014.02.002
Yakymenko I., Tsybulin O., Sidorik E., Henshel D., Kyrylenko O., Kyrylenko S. Oxidative mechanisms of biological activity of low-intensity radiofrequency radiation. Electromagnetic Biology and Medicine. 2015. V. 19. P. 1–16. doi: 10.3109/15368378.2015.1043557
Shakhbazov V.G., Shkorbatov Iu.G., Grabina V.A. Vliianie elektromagnitnogo izlucheniia millimetrovogo diapazona na elektrokineticheskie svoystva khromatina i kletochnykh iader. Molekuliarnaia genetika i biofizika. 1992. No. 16. P. 30–33. [in Russian]
Nylund R., Tammio H., Kuster N., Leszczynski D. Proteomic analysis of the response of human endothelial cell line EA.hy926 to 1800 GSM mobile phone radiation. Journal of Proteomics & Bioinformatics. 2009. V. 2 (10). P. 455–462. doi: 10.4172/jpb.1000105
Shakina L.A., Pasiuga V.N., Dumin O.M., Shckorbatov Yu.G. Effects of microwaves on the puffing pattern of D. melanogaster. Central Europen Journal of Biology. 2011. V. 6 (4). Р. 524–530. doi: 10.2478/s11535-011-0032-x
Arking R. Strategies for stage-specific extension of longevity. In: Life Extension, Healthy Ageing and Longevity, Life Extension: Lessons from Drosophila / A.M. Vaiserman et al. (eds.). Switzerland: Springer International Publishing, 2015. V. 3. Р. 321–347. doi: 10.1007/978-3-319-18326-8_1