CDDP markers MYB and MYC spectra variability in introgressive wheat lines

  • A. G. Navalikhina National University of Kyiv-Mohyla Academy, Ukraine, 04655, Kyiv, Skovorody str., 2
  • M. Z. Antonyuk National University of Kyiv-Mohyla Academy, Ukraine, 04655, Kyiv, Skovorody str., 2
  • T. K. Ternovska National University of Kyiv-Mohyla Academy, Ukraine, 04655, Kyiv, Skovorody str., 2
DOI: https://doi.org/10.7124/FEEO.v20.737

Abstract

Aim. Genes that control the development of glume hairiness and color in wheat (Hg and Rg) were identified decades ago. However, molecular genetics of these traits development is still unknown. Conservative regulatory pathway for color and hairiness development in plants involve transcription factors that belong to MYB and MYC families. Presumably, wheat color and hairiness genes encode these transcription factors. Emergence of dark-colored hairy plants in introgressive wheat lines derived from light-colored glabrous parents may result from the rearrangements in the genes that encode MYB or MYC transcription factors. Methods. Rearrangements in MYB and MYC genes were identified by CDDP markers. Results. New components were identified mainly in MYC CDDP spectra of introgressive lines – derivatives of Aurosis and Aurotica. Only one new component was identified in MYB CDDP spectra of two Aurosis lines. However, it was noticed that none of new components are associated with nonparental phenotype. Besides, CDDP markers have low reproducibility, and so, new components may result from nonspecific amplification. Conclusion. New components in the CDDP markers spectra MYB and MYC may not be associated with rearrangements in corresponding genes due to their minority and low method reproducibility.
Keywords: glume color, glume hairiness, introgressive lines, wheat.

References

Himi E., Nisar A., Noda K. Colour genes (R and Rc) for grain and coleoptile upregulate flavonoid biosynthesis genes in wheat. Genome. 2005. V. 4. P. 747-754. doi: 10.1139/g05-026

Khlestkina E.K., Röder M.S., Börner A. Mapping genes controlling anthocyanin pigmentation on the glume and pericarp in tetraploid wheat (Triticum durum L.). Euphytica. 2009. V. 1. P. 65-69. doi: 10.1007/s10681-009-9994-4

Albert N.W., Davies K.M., Lewis D.H. A conserved network of transcriptional activators and repressors regulates anthocyanin pigmentation in eudicots. Plant Cell. 2014. V. 3. P. 962-980. doi: 10.1105/tpc.113.122069

Himi E., Noda K. Red grain colour gene (R) of wheat is a Myb-type transcription factor. Euphytica. 2005. V. 3. P. 239-242. doi: 10.1007/s10681-005-7854-4

Khlestkina E.K., Shoeva O.Y., Gordeeva E.I. Flavonoid biosynthesis genes in wheat. Russ. J. Genet. Appl. Res. 2015. V. 3. P. 268-278. doi: 10.1134/S2079059715030077

Khlestkina E.K. Geny, determiniruiushchie okrasku razlichnykh organov pshenitsy. Vavilovskiy zhurnal genetiki i selektsii. 2012. No. 1. P. 202-216. [in Russian]

Ishida T., Kurata T., Okada K. A Genetic Regulatory Network in the Development of Trichomes and Root Hairs. Annu. Rev. Plant Biol. 2008. V. 1. P. 365-386. doi: 10.1146/annurev.arplant.59.032607.092949

Szymanski D.B., Lloyd A.M., Marks M.D. Progress in the molecular genetic analysis of trichome initation and morphogenesis in Arabidopsis. Trends in Plant Science. 2000. V. 5. P. 214-219. doi: 10.1016/S1360-1385(00)01597-1

Zhao M. Regulation of Arabidopsis Trichome Patterning and Anthocyanin Biosynthesis by the TTG1-bHLH-MYB Complex: dissertation for the Degree of Doctor of Philosophy. 2007. 99 p.

Guan X. miR828 and miR858 regulate homoeologous MYB2 gene functions in Arabidopsis trichome and cotton fibre development. Nat. Commun. 2014. V. 5. P. 3050. doi: 10.1038/ncomms4050

Tominaga-Wada R., Nukumizu Y., Sato S. Control of Plant Trichome and Root-Hair Development by a Tomato (Solanum lycopersicum) R3 MYB Transcription Factor. PLOS ONE. 2013. V. 8. P. 1-12. doi: 10.1371/journal.pone.0054019

Zheng K. Ectopic expression of R3-MYB transcription factor gene OsTCL1 in Arabidopsis, but not rice, affects trichome and root hair formation. Scientific Reports 2016. V. 6. P. 1-12. doi: 10.1038/srep19254

Xiao K., Mao X., Lin Y. Trichome, a Functional Diversity Phenotype in Plant. Molecular Biology. 2017. V. 6. P. 1-6. doi: 10.4172/2168-9547.1000183

McIntosh R., Yamazaki Y. Catalogue of gene symbols for wheat. Int. Wheat Genet. Symp. 2008. P. 1-197.

Li X., Duan X., Jiang H. Genome-Wide Analysis of Basic Helix-Loop-Helix Transcription Factor Family in Rice and Arabidopsis. Plant Physiol. 2006. V. 141. P. 1167-1184. doi: 10.1104/pp.106.080580

Heim M.A., Jakoby M., Werber M. The basic helix-loop-helix transcription factor family in plants: A genome-wide study of protein structure and functional diversity. Mol. Biol. Evol. 2003. V. 5. P. 735-747. doi: 10.1093/molbev/msg088

Sambrook J., Russell D.W. Molecular Cloning. New York. 2001. 2231 p.

Collard B.C.Y., Mackill D.J. Conserved DNA-derived polymorphism (CDDP): A simple and novel method for generating DNA markers in plants. Plant Mol. Biol. Report. 2009. V. 4. P. 558-562. doi: 10.1007/s11105-009-0118-z

Marchler-Bauer A., Derbyshire M.K., Gonzales N.R. CDD: NCBI's conserved domain database. Nucleic Acid Res. 2015. V. 43. P. 222-226. doi: 10.1093/nar/gku1221

Zhirov E.G., Ternovskaia T.K. Genomnaia inzheneriia u pshenitsy. Vestnik s.-kh. nauki. 1984. No. 10. P. 58–66. [in Russian]

Primer-BLAST. Available from: https://www.ncbi.nlm.nih.gov/tools/primer-blast/