Agrobacterium-mediated transformation of the basal portion of wheat seadling

Abstract

Aims. The aim of our work was to study the effectiveness of the basal portion of wheat seedlings as explants for Agrobacterium-mediated transformation. Methods. We has been used the method of Agrobacterium-mediated transformation and PCR to display results. Results. As a result of genetic transformation we obtained resistant to kanamycin plants. Transgenic status of the four obtained forms was confirmed by PCR. Conclusions. The data suggest that basal portion of wheat seedlings can be used for Agrobacterium-mediated transformation of wheat.

Key words: Triticum aestivum L., Agrobacterium-mediated transformation, wheat.