Analysis of the transgenes inheritance in the genetically modified wheat plants with proline metabolism genes

Abstract

Aim. Analysis of the marker and target genes inheritance in the progeny of transgenic wheat plants with proline metabolism genes. Methods. Agrobacteium-mediated transformation in vitro, genetic and statistical analysis. Results. Progeny segregation analysis indicated that the transgenes were integrated into the same locus of the wheat genome, resulting in a 3:1 segregation ratio of the transgenes. Expression analysis of all transgenes revealed that the transgenes were expressed in all generations (T0-T2). Conclusions. Homozygous progeny plants expressing the marker and target genes were obtained in the T2 generation of lines Zymoyarka 1 and Zymoyarka 154.
Keywords: Triticum aestivum, Agrobacterium-mediated transformation, inheritance of transgenes.