Synergystic effect of antibiotics timentin and ceftriaxone at regeneration in vitro of bread wheat Triticum aestivum

Abstract

Aim. The main requirement for antibiotics used in Agrobacterium-mediated transformation is the absence of toxic effects on plant organisms. The purpose of study is to determine the synergistic action of β-laktam antibiotics on in vitro regeneration of bread wheat T. aestivum. Methods. 18-day calli obtained from apical meristem of wheat, were used as explants to determine the effect of antibiotics on regeneration in vitro. Calli were placed in Petri dishes (50 pcs.) with the regeneration culture medium MS supplemented with 0.5 mg/l of 6-benzylaminopurine (BAP), 0.15 mg/l of picloram, 350 mg/l Timentin and 25–400 mg/l of Ceftriaxone. As a control, culture medium MS supplemented with 0.5 mg/l BAP and 0.15 mg/l picloram without adding antibiotics was used. Cultivation was conducted at 24°C and 16 hours photoperiod for 30 days. The results were processed statistically using Microsoft Excel. Results. It was established that the combination of Timentin (350 mg/l) and Ceftriaxone (350 mg/l) in culture medium for both studied wheat genotypes (Zymoyarka and Podolyanka) stimulates the formation of the maximum number of shoots, for Zymoyarka – 32.4 % (control – 26.5 %), and for Podolyanka – 37.8 % (control – 25.5 %). Conclusions. The synergic effect of antibiotics has a genotype-dependent character. The combination of high concentrations of Timentin (350 mg/l) and Ceftriaxone (350 mg/l) in the culture medium increases the frequency of regeneration of wheat and can be efficiently used during Agrobacterium-mediated wheat transformation in vitro.

Keywords: wheat, antibiotics, regeneration, Timentin, Ceftriaxone.