Introduction of some barley accessions to in vitro culture introduced from international centre ICARDA

Abstract

Aim. From ICARDA in Azerbaijan barley seed nursery introduced with accessions of different purposes. Along with field tests to identify economically valuable genotypes, studies conducted under in vitro technology. The aim of this work was to obtain cell cultures of a number of different accessions of different seed nursery, introduced from ICARDA.Methods.In vitro methods for the induction of callusogenesis and organogenesis of barley are used. Results.When used as explants of mature embryos callus differences between speed and late-ripening varieties have not been identified. In embodiments withetiolated germs, callusogenesis was observed in all the samples studied, and the proliferation process was more intense.Conclusions.The obtained data showed a rigid dependence of callusogenesis processes on the genotype and explant used. For comparison of the adaptive characteristics of a particular nursery with the ability to grow in vitro conditions it is necessary to carry out experiments with a large number of accesions in each individual nursery.

Keywords:barley,callusogenesis, mature corcule, etiolated germs, ICARDA.