The second intron length polymorphism of actin genes in Linum usitatissimum L. genome

Abstract

Aim. In recent years many promising new alternative molecular marker techniques have been developed in plant genetics. Molecular markers may be generated from functional and/or transcribed regions of the genome using different gene-targeting approaches. Recent reports have shown intron length polymorphism (ILP) to be a convenient and reliable source of information with high interspecies and intraspecies variability. Methods. PCR amplification and CTAB-method for the isolation of DNA were used. Amplification products were divided by electrophoresis in 6 % polyacrylamide gel and visualized using silver nitrate method. Results. 10 varieties of flax (Linum иsitatissimum L.) were analysed. The presence of intron length polymorphism has been shown in one of the actin genes. That is gene Lus10021057. It was found that the number of the amplicons that correspond the second intron of actin varying within 780 to 820 bp. Conclusions. The data obtained show that the intron length polymorphism of actin genes can be used for the detection of genetic diversity in different taxa of plants.

Keywords: molecular marker, actin, (tubulin base polimorphism), ILP (intron length polymorphism).