Seed surface sterilization efficiency as an important prerequisite in formation of endangered plant species in vitro collections

Abstract

Aims. Methods of in vitro conservation offer a number of advantages for endangered species preservation. The aim of this research was to elaborate efficient protocols of seed surface sterilization for several plant species of the Red Book of Ukraine taking into account the limited quantity of starting plant material. Methods. Comparison of surface sterilization methods using different antiseptic compounds has been carried out in combination with some stratification approaches. Results. For eight endangered plant species the efficient protocols of surface sterilization were elaborated. Conclusions. The influence of different antiseptic compounds on plant seed germination and the ways of improvement the surface sterilization process are shown.

Keywords: threatened species, biological diversity, seed surface sterilization.