The influence of mannitol on induction and regeneration of morphogenic callus tissue in maize

Abstract

Aims. In maize (Zea mays L.) callusogenesis on immature embryos is the basis for obtaining somaclones, cell selection, genetic engineering. The aim of the study was to examine the effect of mannitol in an induction medium on obtaining and regeneration capacity of morphogenic calli. Methods. Plant tissue culture method for assessing morphogenetic reaction of 9 maize lines was used. Results. Mannitol significantly increased frequencies of morphogenic calli induction, reduced their differentiation, but inhibited fresh weight accumulation. Calli obtained on the mannitol contained medium had improved regeneration ability. Conclusions. Mannitol is an effective component for the induction of morphogenic calli of different types and for maintenance of regeneration ability of callus tissue of type I.

Keywords: mannitol, maize, culture in vitro, morphogenic callus, regenerants.