Development of methodological approaches to obtaining of exosome-like nanovesicles from Urtica dioica L.

DOI: https://doi.org/10.7124/FEEO.v37.1770
Keywords: exosomes, exosome-like nanovesicles of plant origin, isolation methods, Urtica dioica, dynamic light scattering, transmission electron microscopy, ultracentrifugation

Abstract

Aim. To develop methodological approaches for the effective isolation of exosome-like nanovesicles from dioecious nettle (Urtica dioica L.) plants, taking into account their purity, quantity and primary characteristics. Methods. The preparation of exosome-like nanovesicles from the green biomass of nettle and their characterization was carried out using mechanical and cryo-homogenization, centrifugation, dynamic light scattering, and transmission electron microscopy. Results. As a result of this work, conditions were selected, exosome-like nanovesicles were isolated from the green biomass of dioecious nettle by various methods, and their primary characterization was carried out. Conclusions. The suitability of the tested methods for the preparation of exosome-like nanovesicles from green biomass of U. dioica was demonstrated. The exosome-like nanovesicles obtained by different methods and their modifications are quite similar in size and structure. Certain differences in the results of the studies using dynamic light scattering and transmission electron microscopy are within the limits of the methods reproducibility.

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