Features of cellular selection of tomato plants, resistance against salining

  • R. V. Kovbasenko Institute of Cell Biology and Genetic Engineering of Nat. Acad. Sci. of Ukraine, Ukraine, 03143, Kyiv, Akad. Zabolotnoho str., 148 https://orcid.org/0000-0002-0774-362X
  • A. P. Kravets Institute of Cell Biology and Genetic Engineering of Nat. Acad. Sci. of Ukraine, Ukraine, 03143, Kyiv, Akad. Zabolotnoho str., 148 https://orcid.org/0000-0002-4979-5022
  • Yu. V. Symonenko Institute of Cell Biology and Genetic Engineering of Nat. Acad. Sci. of Ukraine, Ukraine, 03143, Kyiv, Akad. Zabolotnoho str., 148 https://orcid.org/0000-0002-5597-3315
  • V. I. Emelyanov Institute of Cell Biology and Genetic Engineering of Nat. Acad. Sci. of Ukraine, Ukraine, 03143, Kyiv, Akad. Zabolotnoho str., 148; Institute of Plant Physiology and Genetics of Nat. Acad. Sci. of Ukraine, Ukraine, 03022, Kyiv, Vasylkivska str., 31/17 https://orcid.org/0009-0003-5216-3207
DOI: https://doi.org/10.7124/FEEO.v34.1635
Keywords: Lycopersicon esculentum Mill., cell selection, antioxidant enzymes, growth regulators, salting

Abstract

Aim. For selection at the cellular level, nutrient media are offered, which, although not completely, would partially correspond to natural stress conditions. Methods. As explants, immature embryos, cotyledon nodes with meristematic activity, and hypocotyls of tomato plants of the Lagidnyi, Khoryv, Borivskyi. Results. An agar medium of general mineral composition with different concentrations of growth regulators was used for the cultivation of isolated tissues the generally accepted method. Conclusions. When creating in vitro new forms of tomato plants with increased resistance to chloride salinization of the soil, it is necessary to select resistant cell lines using the stepwise selection method according to the scheme, which includes several consecutive actions of a selective agent in different concentrations at the stages of induction – proliferation, morphogenesis of callus cultures. An increase in the content of hydrogen peroxide in callus structures and stimulation of the activity of antioxidant enzymes superoxide dismutase and peroxidase were also found.

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