Characteristics of lectin receptors on erythrocyte membranes of patients with glioblastoma

  • I. S. Karpova Institute of Molecular Biology and Genetics, NAS of Ukraine, Ukraine, 03143, Kyiv, Akad. Zabolotnogo str., 150 https://orcid.org/0000-0002-8472-6452
  • I. M. Shuba The State Institution "Romodanov Neurosurgery Institute, NAMS of Ukraine", Ukraine, 04050, Kyiv, Mayborody str., 32 https://orcid.org/0000-0003-4385-4877
  • V. V. Lylo Institute of Molecular Biology and Genetics, NAS of Ukraine, Ukraine, 03143, Kyiv, Akad. Zabolotnogo str., 150
  • O. Y. Glavatskyi The State Institution "Romodanov Neurosurgery Institute, NAMS of Ukraine", Ukraine, 04050, Kyiv, Mayborody str., 32 https://orcid.org/0000-0003-0889-9762
DOI: https://doi.org/10.7124/FEEO.v34.1634
Keywords: panel of lectins, glioblastoma, red blood cells, unidirectional deviations

Abstract

Aim. This work is devoted to the search the abnormalities of the receptor-lectin reactions associated with glioblastoma for diagnostic purposes. Methods. Red blood cell (RBC) specimens were taken from 22 persons distributed into the two cohorts: donors without an oncological diagnosis; patients with glioblastoma (GB). Panel of 9 commercial lectins (LECTINOTEST, Lviv, Ukraine) was used: ConA, WGA, PHA-P; PHA-E; PHA-L, SNA, VAA, STA, and LABA. Also the original lectin from persimmon sepals (named PSL) was used. It was obtained by isoelectric focusing and ammonium sulfate fractionation. Intensity of lectin-receptor interactions was determined in reaction of hemagglutination. Results. Тwo lectins showed some directed divergence in patients from donors. For wheat germ agglutinin (WGA) сhanges in the intensity of lectin-receptor binding were downward. In the case of persimmon L (PSL) the alternative result was observed: the intensity of hemagglutination showed a trend to exceed the level of control. Conclusions. Some individual features in the reaction of erythrocytes with a set of lectins are observed in all examined persons. For two studied lectins, namely WGA and PSL, unidirectional deviations were observed in the reaction of erythrocytes of patients with GB compared to individuals without an oncological diagnosis.

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